Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer

Buffered Saline Solution for Antibody Staining and Wash Steps in Flow Cytometry

Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer is a ready-to-use, optimized buffer formulated for use in immunofluorescent staining protocols involving cells in suspension. It is ideal for antibody dilution, cell suspension preparation, and wash steps during surface and intracellular staining for flow cytometric analysis.

This buffer contains fetal bovine serum (FBS) and 0.09% sodium azide to help preserve samples and reduce nonspecific binding, ensuring cleaner, more reproducible data.

Key Features and Benefits

• Ready-to-Use Convenience
  Supplied as a pre-formulated, isotonic solution—no dilution or mixing required.
• Minimizes Background Staining
  Fetal bovine serum helps block Fc receptors and reduce nonspecific antibody binding.
• Supports All Steps of Flow Cytometry Staining
  Use for antibody dilution, cell washes, and resuspension before sample acquisition.
• Stable and Reproducible
  Contains sodium azide (0.09%) for preservative action and long-term stability.

Applications

• Flow cytometry sample preparation
• Surface and intracellular antibody staining protocols
• Immunophenotyping and multiparameter cell analysis
• Cell wash steps in fixation/permeabilization workflows

Technical Specifications

Invitrogen™ eBioscience™ Flow Cytometry Staining Buffer is a reliable, serum-containing buffer optimized for clean, low-background staining in flow cytometry workflows. Its compatibility with both surface and intracellular staining makes it a valuable tool in immunophenotyping, immune monitoring, and single-cell analysis.