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Invitrogen™ eBioscience™, CD184 (CXCR4) Monoclonal Antibody (12G5), PE
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Primary Antibodies | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The CD184 (CXCR4) Monoclonal Antibody, Clone 12G5, PE-conjugated, is a reliable and widely cited reagent designed for high-sensitivity flow cytometric analysis of human CXCR4 expression. CXCR4, a chemokine receptor also known as Fusin, is involved in diverse biological processes, including T cell trafficking, hematopoiesis, and HIV-1 entry into CD4+ cells.
This PE-labeled antibody provides bright, photostable fluorescence suitable for multicolor flow cytometry and intracellular staining applications. Clone 12G5 is especially well-suited for immune profiling, stem cell characterization, and viral receptor studies.
Key Features and Benefits
- Highly Specific: Recognizes the human CD184 (CXCR4) receptor with high affinity.
- PE Conjugation: Offers strong fluorescence for accurate detection and analysis.
- Clone 12G5: Well-characterized and validated in over 30 publications.
- Pre-titrated Format: Ready to use—5 µL per test in 100 µL total staining volume.
- Flow Cytometry-Optimized: Suitable for use with blue, green, or yellow-green lasers.
Application
- Primary Application: Flow Cytometry (5 µL/test)
- Also Reported For: Immunohistochemistry (1 publication)
Specifications
| Attribute | Details |
|---|---|
| Clone | 12G5 |
| Reactivity | Human |
| Host / Isotype | Mouse / IgG2a, κ |
| Conjugate | PE (Phycoerythrin) |
| Excitation / Emission | 565 nm / 576 nm |
| Form | Liquid |
| Volume per Test | 5 µL |
| Storage Buffer | PBS, pH 7.2, with BSA and 0.09% NaN₃ |
| Storage Conditions | 4°C, protect from light, DO NOT FREEZE |
| Shipping Conditions | Ambient (domestic), Wet ice (international) |
| Purification | Affinity chromatography |
| Filter | 0.2 µm post-manufacturing |
| RRID | AB_10669164 |
Target Information: CXCR4 (CD184)
CXCR4 is a seven-transmembrane G-protein-coupled receptor (GPCR) that acts as a coreceptor for X4-tropic HIV-1 and HIV-2. It binds to CXCL12/SDF-1, mediating cell migration, chemotaxis, and calcium signaling. CXCR4 is widely expressed on naive T cells, hematopoietic progenitor cells, and various tumor cells, making it a crucial marker in immunology, virology, stem cell biology, and cancer research.
The Invitrogen™ CD184 (CXCR4) PE-Conjugated Antibody, Clone 12G5 is a powerful and validated tool for high-sensitivity flow cytometry, enabling researchers to accurately detect and quantify CXCR4 expression in human cells. Its excellent performance, broad validation, and compatibility with multicolor staining workflows make it an essential reagent for immunophenotyping and disease research.
- Pack Size: For 100 Tests for 25 Tests