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Invitrogen™ eBioscience™ CD184 (CXCR4) Monoclonal Antibody (12G5), APC
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Specifications:
| Application | Flow Cytometry | ||
| Storage Temperature | 2-8°C | ||
| Product Type | antibody | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ CD184 (CXCR4) Monoclonal Antibody (12G5), APC-conjugated is a high-affinity, flow cytometry–validated antibody targeting human CXCR4 (CD184), a chemokine receptor involved in immune signaling, HIV entry, and developmental biology. The 12G5 clone binds to an extracellular epitope of CXCR4, enabling specific detection of surface-expressed CXCR4 on lymphocytes and other immune cell populations.
CXCR4 is a seven-transmembrane G-protein-coupled receptor for stromal-derived factor-1 (SDF-1/CXCL12), and acts as a co-receptor for X4-tropic HIV-1 and HIV-2. Its expression is rapidly upregulated upon immune stimulation and plays a critical role in hematopoiesis, neurogenesis, inflammation, and tumor metastasis.
Key Features and Benefits
- Flow cytometry validated: Optimized and pre-titrated for use in multicolor flow cytometry panels.
- Clone 12G5: Highly cited monoclonal antibody with published specificity for human CXCR4.
- APC-conjugated: Excitable with red laser; ideal for panels requiring far-red fluorophores.
- Inhibitory activity: Reported to block HIV-1 entry and SDF-1–induced signaling.
- Highly specific: Minimal cross-reactivity; suitable for profiling CXCR4 in human PBMCs.
Applications and Tested Dilution
| Application | Tested Dilution | Details |
|---|---|---|
| Flow Cytometry | 5 µL/test (0.25 µg) | For 1 × 10⁵ to 1 × 10⁸ cells in 100 µL |
Technical Specifications
| Parameter | Detail |
|---|---|
| Species Reactivity | Human |
| Published Reactivity | Human, Hamster |
| Clone | 12G5 |
| Antigen Target | CXCR4 (CD184) |
| Host / Isotype | Mouse / IgG2a, κ |
| Conjugate | APC (Allophycocyanin) |
| Excitation/Emission | 651/660 nm |
| Form | Liquid |
| Concentration | 5 µL/test |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.2, with BSA and 0.09% sodium azide |
| Storage Conditions | 4°C, protect from light, Do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| Isotype Control | Mouse IgG2a κ APC (eBM2a) – eBioscience™ |
| Filtration | 0.2 µm filtered post-manufacture |
Biological Background
CXCR4 (CD184) is a chemokine receptor essential for directing the migration of hematopoietic, immune, and neuronal cells. It binds to CXCL12/SDF-1, initiating signaling pathways that regulate cell movement, calcium mobilization, and MAPK activation. CXCR4 plays critical roles in cardiac development, vascular remodeling, and immune cell trafficking. It is a coreceptor for HIV-1 (X4-tropic strains) and a therapeutic target in cancer and inflammation.
The 12G5 antibody has been shown to:
- Partially inhibit SDF-1–induced chemotaxis and calcium signaling
- Block HIV-1 and HIV-2 envelope-mediated fusion
- Stain CXCR4 on T cells, especially naïve subsets in human peripheral blood
The Invitrogen™ CD184 (CXCR4) Monoclonal Antibody (12G5), APC-conjugated, is a powerful tool for detecting, quantifying, and characterizing CXCR4 expression in immune, developmental, and HIV research. Its red-fluorescent conjugation and proven performance in flow cytometry make it ideal for multicolor immune phenotyping, disease modeling, and therapeutic target validation.
- Pack Size: For 100 Tests for 25 Tests