Invitrogen™ CellTracker™ Fluorescent Probe

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Specifications:
Application Cell Analysis
Storage Temperature -5 to -30°C
Product Type Dye, Flow Cytometry Reagents Forms Powder
Product Brand Thermo Fisher Scientific™, Invitrogen
Product Grade Molecular Biology

Long-retention, low-toxicity dyes for live-cell tracking, proliferation, and migration studies

Invitrogen™ CellTracker™ fluorescent probes are a family of cell-permeant, long-lasting dyes designed for live-cell tracking and multiplex imaging in both adherent and suspension cell types. These probes freely diffuse across the plasma membrane, where they are converted into cell-impermeant fluorescent reaction products that are retained through multiple cell divisions (typically three to six generations).

With low cytotoxicity and retention of fluorescence for >72 hours, CellTracker dyes enable long-term studies of cell movement, proliferation, migration, chemotaxis, invasion, and localization. The range of available emission colors ensures compatibility with common lasers and filter sets for single or multicolor experiments, including co-staining with antibodies and other probes.

Available Dyes & Spectral Properties

Catalog No.Dye NameQuantityExcitation / Emission (nm)
C34565CellTracker™ Deep Red20 × 15 μg630 / 660
C2110CellTracker™ Blue CMAC5 mg353 / 466
C2111CellTracker™ Blue CMHC5 mg372 / 470
C12881CellTracker™ Blue CMF2HC5 mg371 / 464
C7025CellTracker™ Green CMFDA20 × 50 μg492 / 517
C2925CellTracker™ Green CMFDA1 mg492 / 517
C2102CellTracker™ Green BODIPY™5 mg522 / 529
C34551CellTracker™ Orange CMRA20 × 50 μg548 / 576
C2927CellTracker™ Orange CMTMR1 mg541 / 565
C34552CellTracker™ Red CMTPX20 × 50 μg577 / 602
C10094CellTracker™ Violet BMQC5 × 0.1 mg415 / 516

Key Features

  • Bright, stable fluorescence for >72 hours
  • Low cytotoxicity – does not interfere with cell viability or proliferation
  • Multi-generational retention – dyes are passed to daughter cells without leakage to adjacent cells
  • Broad color range – compatible with multiple excitation/emission channels for multiplexing
  • Flexible applications – suitable for both adherent and suspension cells
  • Fixation compatibility – most dyes retain fluorescence after fixation and permeabilization for ICC or ISH workflows

Applications

  • Cell tracking in live-cell imaging and microscopy
  • Proliferation studies over multiple generations
  • Migration, chemotaxis, and invasion assays
  • Cell localization in co-culture and mixed population studies
  • Multiplex imaging with antibodies and organelle stains

Specifications

ParameterDetails
Detection MethodFluorescence
FormatDry powder
Storage Conditions–5°C to –30°C, desiccated, protect from light
Cell PermeabilityCell-permeant (converted to cell-impermeant products inside cells)
Retention Time≥72 hours
Regulatory StatusResearch Use Only (RUO)


Invitrogen™ CellTracker™ fluorescent probes provide bright, stable, and nontoxic labeling for live-cell tracking and long-term imaging studies. With a wide spectral range and high retention, these probes are ideal for cell motility, proliferation, and localization experiments, as well as for fixed-cell multiplex immunostaining workflows.

  • Label or Dye:   CellTracker™ Deep Red, 20 x 15 μg CellTracker™ Blue CMAC, 5mg CellTracker™ Blue CMHC, 5mg CellTracker Blue CMF2HC, 5mg CellTracker Green CMFDA, 20 x 50 μg CellTracker Green CMFDA, 1mg CellTracker Green BODIPY, 5mg CellTracker Orange CMRA, 20 x 50 μg CellTracker Orange CMTMR, 1mg CellTracker™ Red CMTPX, 20 x 50 μg CellTracker Violet BMQC, 5 x 0.1 mg

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