Invitrogen™ CellTracker™ Fluorescent Probe
Catalog No :
CAS Number :
Brand :
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Specifications:
Application | Cell Analysis | ||
Storage Temperature | -5 to -30°C | ||
Product Type | Dye, Flow Cytometry Reagents | Forms | Powder |
Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
Product Grade | Molecular Biology | ||
Long-retention, low-toxicity dyes for live-cell tracking, proliferation, and migration studies
Invitrogen™ CellTracker™ fluorescent probes are a family of cell-permeant, long-lasting dyes designed for live-cell tracking and multiplex imaging in both adherent and suspension cell types. These probes freely diffuse across the plasma membrane, where they are converted into cell-impermeant fluorescent reaction products that are retained through multiple cell divisions (typically three to six generations).
With low cytotoxicity and retention of fluorescence for >72 hours, CellTracker dyes enable long-term studies of cell movement, proliferation, migration, chemotaxis, invasion, and localization. The range of available emission colors ensures compatibility with common lasers and filter sets for single or multicolor experiments, including co-staining with antibodies and other probes.
Available Dyes & Spectral Properties
Catalog No. | Dye Name | Quantity | Excitation / Emission (nm) |
---|---|---|---|
C34565 | CellTracker™ Deep Red | 20 × 15 μg | 630 / 660 |
C2110 | CellTracker™ Blue CMAC | 5 mg | 353 / 466 |
C2111 | CellTracker™ Blue CMHC | 5 mg | 372 / 470 |
C12881 | CellTracker™ Blue CMF2HC | 5 mg | 371 / 464 |
C7025 | CellTracker™ Green CMFDA | 20 × 50 μg | 492 / 517 |
C2925 | CellTracker™ Green CMFDA | 1 mg | 492 / 517 |
C2102 | CellTracker™ Green BODIPY™ | 5 mg | 522 / 529 |
C34551 | CellTracker™ Orange CMRA | 20 × 50 μg | 548 / 576 |
C2927 | CellTracker™ Orange CMTMR | 1 mg | 541 / 565 |
C34552 | CellTracker™ Red CMTPX | 20 × 50 μg | 577 / 602 |
C10094 | CellTracker™ Violet BMQC | 5 × 0.1 mg | 415 / 516 |
Key Features
- Bright, stable fluorescence for >72 hours
- Low cytotoxicity – does not interfere with cell viability or proliferation
- Multi-generational retention – dyes are passed to daughter cells without leakage to adjacent cells
- Broad color range – compatible with multiple excitation/emission channels for multiplexing
- Flexible applications – suitable for both adherent and suspension cells
- Fixation compatibility – most dyes retain fluorescence after fixation and permeabilization for ICC or ISH workflows
Applications
- Cell tracking in live-cell imaging and microscopy
- Proliferation studies over multiple generations
- Migration, chemotaxis, and invasion assays
- Cell localization in co-culture and mixed population studies
- Multiplex imaging with antibodies and organelle stains
Specifications
Parameter | Details |
---|---|
Detection Method | Fluorescence |
Format | Dry powder |
Storage Conditions | –5°C to –30°C, desiccated, protect from light |
Cell Permeability | Cell-permeant (converted to cell-impermeant products inside cells) |
Retention Time | ≥72 hours |
Regulatory Status | Research Use Only (RUO) |
Invitrogen™ CellTracker™ fluorescent probes provide bright, stable, and nontoxic labeling for live-cell tracking and long-term imaging studies. With a wide spectral range and high retention, these probes are ideal for cell motility, proliferation, and localization experiments, as well as for fixed-cell multiplex immunostaining workflows.
- Label or Dye: CellTracker™ Deep Red, 20 x 15 μg CellTracker™ Blue CMAC, 5mg CellTracker™ Blue CMHC, 5mg CellTracker Blue CMF2HC, 5mg CellTracker Green CMFDA, 20 x 50 μg CellTracker Green CMFDA, 1mg CellTracker Green BODIPY, 5mg CellTracker Orange CMRA, 20 x 50 μg CellTracker Orange CMTMR, 1mg CellTracker™ Red CMTPX, 20 x 50 μg CellTracker Violet BMQC, 5 x 0.1 mg