Invitrogen™ CellTrace™ CFSE Cell Proliferation Kit, for flow cytometry

The CellTrace™ CFSE Cell Proliferation Kit provides a reliable, bright, and non-toxic method for tracking cell divisions in both in vitro and in vivo studies. By utilizing dye dilution analysis via flow cytometry, this kit enables clear visualization of up to eight or more generations of proliferating cells.

Key Features

• High-Resolution Proliferation Tracking: Bright, single-peak fluorescence enables clear resolution of multiple cell generations.
• Long-Term Signal Stability: The dye covalently binds inside the cell, ensuring retention for several days under culture conditions.
• Low Cytotoxicity: Does not affect cell viability or proliferation capacity.
• Easy-to-Use Protocol: Rapid staining with simple steps and ready-to-use lyophilized vials.

Applications

• Flow cytometry-based cell proliferation tracking
• Immune response studies and lymphocyte tracking
• Assessment of hematopoietic cell division
• Longitudinal tracking of transplantable cells in animal models

How It Works

CellTrace™ CFSE (carboxyfluorescein diacetate succinimidyl ester) diffuses into live cells and binds covalently to intracellular proteins. Upon each cell division, the dye is equally distributed between daughter cells, resulting in a halved fluorescence intensity—this enables the monitoring of sequential cell divisions using flow cytometry.

Kit Components

• 10 × vials of CellTrace™ CFSE (lyophilized powder)
• 1 × 500 μL vial of anhydrous DMSO

Staining Protocol Highlights

1. Reconstitute dye in DMSO.
2. Incubate cells (in protein-free media) with 1 µL dye per 1 mL cell suspension for 20 minutes at room temperature.
3. Quench excess dye with complete medium.
4. Analyze via flow cytometry using 488 nm excitation and 517 nm emission settings.

Specifications

Ordering Information

The CellTrace™ CFSE Cell Proliferation Kit is a trusted solution for researchers seeking reliable and reproducible data in proliferation assays. Whether analyzing immune responses, evaluating drug effects on cell division, or tracing stem cell expansion, this kit offers superior brightness, consistency, and ease of use.