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Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate)
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Specifications:
| Application | Western Blot, Southern Blot, Northern Bloth | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Concentrated, high-sensitivity chemiluminescent substrate for alkaline phosphatase detection
The Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate) is a high-performance chemiluminescent substrate for alkaline phosphatase (AP), delivering exceptional sensitivity, rapid signal generation, and long-lasting glow emission. Suitable for both membrane-based and solution-based assays, this substrate enables clear detection of AP and AP-labeled molecules with superior signal-to-noise ratio and low background luminescence.
Optimized for applications including Southern, Northern, and Western blotting, as well as immunoassays, DNA probe assays, enzyme assays, and reporter gene assays, CDP-Star™ achieves maximum light levels in approximately 10 minutes, with emission persisting for several hours—providing flexibility for multiple exposures and reimaging.
Key Features & Benefits
- High Sensitivity – Detects alkaline phosphatase labels with superior intensity and clarity.
- Fast Signal Development – Peak chemiluminescence in ~10 minutes.
- Long-Lasting Glow – Stable light emission for several hours.
- Low Background – Produces clean, high-contrast results.
- Versatile – Suitable for blotting and solution-based enzymatic detection assays.
- Concentrated Format – Allows custom dilution to suit application needs.
Applications
- Southern Blotting – DNA detection with AP-labeled probes.
- Northern Blotting – RNA detection in gene expression studies.
- Western Blotting – Protein detection using AP-conjugated antibodies.
- Solution-Based Assays – Immunoassays, DNA probe assays, enzyme assays, and reporter gene assays.
Technical Specifications
| Property | Details |
|---|---|
| Detection Method | Chemiluminescence |
| Substrate Type | AP (Alkaline Phosphatase) Substrate |
| Substrate | Alkaline Phosphatase |
| Concentration | 12.5 mM |
| Form | Liquid |
| Format | Bottle(s) |
| Maximum Light Output | ~10 minutes |
| Glow Duration | Several hours |
| Applications | Southern, Northern, Western blotting; solution-based assays |
| Product Line | CDP-Star™, NovaBright™ |
| Shipping Condition | Room Temperature |
| Storage | 4°C |
Ordering Information
| Catalog Number | Quantity |
|---|---|
| T2306 | 5 mL |
| T2310 | 200 mL |
| T2308 | 20 mL |
| T2309 | 50 mL |
The Invitrogen™ CDP-Star™ Substrate (12.5 mM Concentrate) provides superior sensitivity, speed, and signal stability for AP-based detection methods. Its concentrated format offers flexibility for various assay types, making it a versatile choice for high-performance molecular biology and immunodetection applications.
- Pack Size: 50 mL 5 mL 200 mL 20 mL