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Invitrogen™ Ambion™ Nuclease-Free Water (not DEPC-Treated)
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DNase-Free, Molecular Biology Grade, RNase-Free
Specifications:
| Application | molecular biology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Invitrogen™ Nuclease-Free Water (not DEPC-treated) is a high-purity water solution designed for molecular biology applications. It undergoes rigorous processing, including deionization, membrane filtration, and autoclaving, ensuring it is free from contaminants like endonucleases, exonucleases, and RNases. This product is ready to use without further preparation and is suitable for sensitive applications where residual DEPC might interfere with experimental outcomes.
Key Features
- High Purity and Contaminant-Free:
- Free from detectable levels of nonspecific endonuclease, exonuclease, and RNase activity.
- Tested for molecular biology-grade purity to ensure reliable results.
- Not DEPC-Treated:
- Recommended for applications where DEPC (diethyl pyrocarbonate) residues could be detrimental, such as:
- Oocyte microinjection.
- RNA applications sensitive to DEPC interference.
- Recommended for applications where DEPC (diethyl pyrocarbonate) residues could be detrimental, such as:
- Ready to Use:
- Requires no additional preparation, mixing, or autoclaving.
- Convenient packaging for immediate use in molecular biology workflows.
- Versatile Applications:
- Suitable for use in PCR, qPCR, RNA/DNA preparation, and other molecular biology techniques.
Specifications
| Feature | Details |
|---|---|
| pH | 6 to 8 |
| Grade | Molecular Biology |
| Treatment | Not DEPC-Treated |
| Purification Method | Deionized, membrane-filtered, autoclaved |
| Packaging | Bottle |
| Storage Condition | Room temperature |
| Shipping Condition | Room temperature |
| Product Line | Ambion™ |
Packaging Options
| Catalog Number | Quantity | Unit Size |
|---|---|---|
| AM9939 | 5 x 100 mL | 500 mL total |
| AM9938 | 1 x 100 mL | 100 mL total |
| AM9930 | 1 x 500 mL | 500 mL total |
| AM9937 | 10 x 50 mL | 500 mL total |
| AM9932 | 1 x 1000 mL | 1000 mL total |
| 4387936 | 4 x 1000 mL | 4000 mL total |
Applications
- Molecular Biology Techniques:
- PCR and qPCR assays.
- Preparation of RNA and DNA samples.
- Electrophoresis and related workflows.
- Sensitive Applications:
- RNA analysis or experiments requiring RNase-free conditions.
- Microinjection procedures, such as oocyte manipulation.
- General Laboratory Use:
- Buffer preparation.
- Dilution of reagents and samples.
Advantages
- No Residual DEPC: Ensures compatibility with sensitive RNA or cellular applications.
- High Purity: Reliable for demanding molecular biology workflows.
- Convenience: Pre-autoclaved and ready to use, saving time and reducing contamination risks.
- Flexible Packaging: Available in various sizes to suit different research needs.
Storage and Shipping
- Storage: Room temperature storage ensures ease of use and long shelf life.
- Shipping: Shipped at room temperature for convenience and stability.
Why Choose Invitrogen™ Nuclease-Free Water?
- Designed for reliability and consistency in molecular biology applications.
- Free from DEPC and rigorously tested for contaminating nucleases.
- Ready-to-use format simplifies experimental workflows and minimizes contamination risks.
This nuclease-free water is an essential reagent for any molecular biology lab, offering unparalleled purity and reliability for sensitive experiments.
- Pack Size: 1 x 1,000 ml 4 x 1,000 mL 1 x 500 mL 10 x 50 mL 1 x 100 mL 5 x 100 mL