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Human Suppressor of Cytokine Signaling 3 (SOCS3) ELISA Kit
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Grade | Molecular Biology | ||
The Human Suppressor of Cytokine Signaling 3 (SOCS3) ELISA Kit is a highly sensitive sandwich enzyme-linked immunosorbent assay designed for the quantitative determination of Human SOCS3 antigen in biological samples including serum, plasma, cell culture supernatants, tissue lysates, and other biological fluids.
SOCS3 is an important intracellular regulatory protein that belongs to the Suppressor of Cytokine Signaling (SOCS) family, which functions as a negative regulator of cytokine signaling pathways, particularly within the JAK/STAT signaling pathway. SOCS3 plays a critical role in immune regulation, inflammatory responses, metabolic signaling, and tumor biology, making it a valuable biomarker in studies related to immunology, inflammation, cancer research, and metabolic diseases.
This ELISA kit utilizes a highly specific antibody-coated microplate to capture SOCS3 from samples. Following antigen binding and washing steps, a horseradish peroxidase (HRP)-conjugated detection antibody binds to the captured SOCS3 protein. The addition of tetramethylbenzidine (TMB) substrate produces a colorimetric reaction proportional to the SOCS3 concentration, which is measured at 450 nm using an ELISA microplate reader.
Key Features
- High Sensitivity: Detection limit of 0.5 pg/mL
- Wide Detection Range: 3.1 pg/mL – 200 pg/mL
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High Precision
- Intra-assay CV: <10%
- Inter-assay CV: <12%
- Reliable Sandwich ELISA format
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Compatible with multiple biological samples
- Serum
- Plasma
- Urine
- Cell culture supernatant
- Tissue homogenates
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Flexible Kit Formats
- 48 Test Kit
- 96 Test Kit
- Colorimetric detection at 450 nm
- Simple and standardized workflow
Assay Principle (Sandwich ELISA)
- Microplate wells are pre-coated with antibodies specific to Human SOCS3.
- SOCS3 antigen present in standards or samples binds to the immobilized antibodies.
- After washing, an HRP-conjugated detection antibody binds to the captured SOCS3 antigen.
- Addition of TMB chromogenic substrate results in a blue color reaction.
- The reaction is terminated using an acid stop solution, producing a yellow color.
- The intensity of the color is proportional to the SOCS3 concentration and is measured at 450 nm.
- Sample concentrations are calculated by comparing OD values to the standard curve.
Assay Workflow
Sample / Standard Addition
↓
Incubation (30 min at 37 °C)
↓
Plate Washing (5×)
↓
HRP-Conjugate Addition
↓
Incubation (30 min at 37 °C)
↓
Plate Washing (5×)
↓
TMB Substrate Reaction (15 min)
↓
Stop Solution Addition
↓
OD Measurement at 450 nm
↓
Concentration Determination using Standard Curve
Kit Components
| Component | 96 Tests | 48 Tests | Storage |
|---|---|---|---|
| Instruction Manual | 1 | 1 | RT |
| Adhesive Films | 2 | 2 | RT |
| Sealed Foil Bag | 1 | 1 | RT |
| SOCS3 Antibody-Coated Microplate | 1 | 1 | 2–8 °C |
| SOCS3 Standard (270 pg/mL) | 0.5 mL | 0.5 mL | 2–8 °C |
| Standard Diluent | 1.5 mL | 1.5 mL | 2–8 °C |
| HRP-Conjugate Reagent | 6 mL | 3 mL | 2–8 °C |
| Sample Diluent | 6 mL | 3 mL | 2–8 °C |
| Chromogen Solution A | 6 mL | 3 mL | 2–8 °C |
| Chromogen Solution B | 6 mL | 3 mL | 2–8 °C |
| Stop Solution | 6 mL | 3 mL | 2–8 °C |
| Wash Buffer Concentrate | 20 mL (30×) | 20 mL (20×) | 2–8 °C |
Performance Specifications
| Parameter | Specification |
|---|---|
| Detection Range | 3.1 – 200 pg/mL |
| Sensitivity | 0.5 pg/mL |
| Intra-Assay Precision | CV <10% |
| Inter-Assay Precision | CV <12% |
| Detection Method | Sandwich ELISA |
| Detection Wavelength | 450 nm |
Applications
The Human SOCS3 ELISA Kit is suitable for research applications such as:
- Cytokine signaling pathway research
- JAK/STAT signaling studies
- Immunology and inflammation research
- Cancer biology and tumor microenvironment studies
- Metabolic disease research
- Biomarker discovery and validation
- Drug discovery and therapeutic development
- Cellular signaling pathway analysis
The Human SOCS3 ELISA Kit provides a sensitive, reliable, and quantitative method for measuring SOCS3 protein levels in a wide range of biological samples. With its high sensitivity, broad dynamic detection range, and robust sandwich ELISA format, this kit enables researchers to accurately investigate SOCS3 involvement in cytokine signaling, immune regulation, inflammation, and disease pathogenesis. Its standardized reagents, simple workflow, and compatibility with common laboratory equipment make it a valuable tool for immunology, oncology, metabolic research, and biomarker discovery studies.
- Pack Size: For 48 Tests For 96 Tests