GoTaq® Probe qPCR Master Mix

Convenient Master Mixes for Probe-Based Real-Time qPCR and RT-qPCR

• Ready-to-use mix of optimized components for robust qPCR and RT-qPCR using hydrolysis probes
• Compatible with fast and standard cycling methods on most real-time PCR instruments using TaqMan® and other probe assays such as molecular beacons
• Resistant to most PCR inhibitors

Simple, Sensitive Probe-Based qPCR and RT-qPCR

The GoTaq® Probe qPCR and RT-qPCR Systems are ready-to-use 2X master mixes that simplify reaction assembly for qPCR using hydrolysis probe detection. These real-time qPCR systems are designed for sensitive detection and quantification of a broad range of DNA or RNA targets in the presence of a wide range of PCR inhibitors.

Rapid hot-start activation and processive enzymes make the GoTaq® Probe qPCR and RT-qPCR Systems compatible with most real-time PCR instruments using standard or fast cycling.  Convenient, room-temperature setup makes it easy to scale up to automated and high-throughput detection formats.

Sensitivity and Specificity for qPCR

The GoTaq® Probe qPCR Master Mix is a ready-to-use, stabilized, 2X formulation with all of the components for quantitative PCR (qPCR). Just add template, primers and probe. Optimized for fast and reproducible quantitative PCR assays in the hydrolysis probe detection format, the master mix contains GoTaq® Hot Start Polymerase, MgCl2, dNTPs and a proprietary reaction buffer. The master mix does not contain a reference dye; a separate tube of CXR reference dye is included.

The GoTaq® Probe qPCR Master Mix offers:

• Sensitive detection for earlier quantitation of low- and high-copy targets over a broad dynamic range.
• Optimized formulation compatible with running multiplex assays.
• Room-temperature setup, making it suitable for automation and high-throughput detection.
• Resistance to a wide range of PCR inhibitors.
  

qPCR amplification from FFPE samples with GoTaq® Probe Master Mix. DNA (10ng) from a lung tumor FFPE sample was amplified under both standard and Fast thermal cycling conditions on a Roche LightCycler 480 instrument using a 384-well plate. Equivalent Cq values were observed for the three genes evaluated regardless of cycling conditions used for the experiment.

Performance Tested in qPCR

We have generated a large collection of data using different instruments, templates, and sample types. Visit the qPCR Data Selector to see the results generated using the GoTaq® qPCR systems.

Suitable for Different Organisms and Sample Types

DNA/RNA isolated from the following organisms and sample types has been used with GoTaq® qPCR Systems. DNA/RNA from organisms and sample types not listed may also be compatible—please enquire with Promega technical support for additional compatibility.

Organism Types:

• Animal
• Bacteria
• Fungi
• Human
• Parasites
• Viruses
• Yeast

Sample Types:

• Blood
• Cells
• Feces
• FFPE
• Food
• Human Cerebrospinal Fluid (CSF)
• Plants
• Plasma
• Saliva
• Serum
• Sputum
• Swabs
• Tissue
• Urine