Gibco™ B-27™ Supplement (50X), Serum-Free

The Gibco™ B-27™ Supplement (50X) is the original, serum-free formulation designed to support the growth, viability, and differentiation of neurons derived from embryonic, postnatal, and adult central nervous system (CNS) tissue.

Manufactured under cGMP and ISO 13485-certified conditions, each lot of B-27 Supplement undergoes rigorous performance testing to confirm its ability to sustain primary hippocampal neuron cultures.

As the first and most-cited neuronal cell culture supplement, B-27 has become the gold standard for reproducible, high-quality serum-free neural culture, enabling consistent results across neurobiology and stem cell differentiation studies.

Key Features

• Serum-Free, Defined Formulation: Ensures consistency, reproducibility, and reduced variability in neuronal cultures.
• Lot-to-Lot Reliability: Every batch is performance-tested for neuron culture support.
• Versatile Compatibility: Optimized for use with Neurobasal™ or Neurobasal™-A media.
• Broad Applicability: Supports neurons, neural stem cells (NSCs), and pluripotent stem cell (PSC)-derived neural lineages.
• High Quality & Compliance: cGMP manufacturing under FDA-registered, ISO 13485-certified facilities in Grand Island, NY.
• Shelf Life: 12 months; store at −5 to −20 °C and protect from light.
• Research Use Only (RUO): Not for diagnostic or therapeutic use.

Specifications

Applications

• Serum-free culture of primary hippocampal and cortical neurons
• Long-term maintenance of CNS and PSC-derived neurons
• Neuronal differentiation from neural stem or progenitor cells
• Use with Neurobasal™ or Neurobasal™-A medium for optimized neuron viability
• Serum-free model development for neurotoxicity and neuroprotection studies

The Gibco™ B-27™ Supplement (50X) remains the industry standard for serum-free neuronal culture, trusted for its proven performance and reproducibility.

Its cGMP production, defined formulation, and broad compatibility with neuronal cell types make it the preferred choice for researchers conducting neurobiology, stem cell differentiation, and neural maintenance studies.