Gibco™ Penicillin-Streptomycin (10,000 U/mL)

The Gibco™ Penicillin-Streptomycin Solution is an essential antibiotic preparation for preventing bacterial contamination in cell cultures. Designed with a high concentration of penicillin (10,000 units/mL) and streptomycin (10,000 μg/mL), this solution offers broad-spectrum antibacterial action, targeting both gram-positive and gram-negative bacteria effectively. It is sterile-filtered for optimal safety and reliability in laboratory applications.

Key Features:

1. Dual Antibiotic Action:
   • Penicillin: Derived from Penicillium fungi, it inhibits bacterial cell wall synthesis by disrupting peptidoglycan turnover, resulting in bacterial lysis.
   • Streptomycin: Sourced from Streptomyces griseus, it binds to the 30S ribosomal subunit, interfering with protein synthesis, leading to bacterial death.
2. Optimal for Cell Culture:
   • Prevents bacterial contamination in mammalian and insect cell cultures.
   • Effective against a wide range of contaminants while ensuring the integrity of the cell culture.
3. Convenient Liquid Form:
   • Ready-to-use liquid format simplifies preparation and reduces handling errors.
   • Supplied as a 100X concentrate for easy dilution into cell culture media.
4. High Sterility Standards:
   • Sterile-filtered to ensure contamination-free usage in sensitive cell culture environments.
5. Storage and Stability:
   • Long-term storage: -5°C to -20°C.
   • Short-term storage: 2–8°C for temporary use.
   • Shelf life: 12 months from the date of manufacture when stored as recommended.

Specifications:

Applications:

• Cell Culture Contamination Control:
  • Ideal for preventing contamination in mammalian, insect, and other cell cultures.
  • Maintains the integrity of sensitive experimental systems.
• Broad-Spectrum Antibacterial Effect:
  • Effective against a wide range of gram-positive and gram-negative bacterial strains.
• Eukaryotic and Bacterial Selection:
  • Suitable for selection experiments in genetic and molecular biology research.

Usage Guidelines:

1. Dilute the 100X concentrate into cell culture media to achieve a working concentration.
2. Maintain sterile conditions to avoid introducing external contaminants.
3. Refer to recommended antibiotic working concentrations for specific cell culture systems.

Storage and Handling:

• Long-term storage: Keep at -5°C to -20°C to maintain potency.
• Short-term storage: Store at 2–8°C for up to 1 month.
• Protect from light and moisture during storage and handling.

Regulatory Information:

• For Research Use Only (RUO): Not intended for diagnostic or therapeutic use.

The Gibco™ Penicillin-Streptomycin solution ensures reliable and effective bacterial contamination prevention in various cell culture systems, making it a staple in research laboratories worldwide.