Gibco™ Advanced MEM

Gibco™ Advanced MEM (Minimum Essential Medium) – Serum-Reduced Mammalian Cell Culture Medium

Optimized for Reduced FBS Supplementation in Mammalian Cell Culture

Gibco™ Advanced MEM (Minimum Essential Medium) is a high-performance basal medium that allows for mammalian cell culture with significantly reduced Fetal Bovine Serum (FBS) supplementation (50-90%), while maintaining normal growth rates and morphology.

This serum-reduced formulation is particularly useful for biopharmaceutical research, cell therapy development, and laboratory-scale culture of various mammalian cell lines, including HeLa, BHK-21, 293, HEP-2, HT-1080, MCF-7, and fibroblasts.

Gibco™ Advanced MEM includes ethanolamine, glutathione, ascorbic acid, insulin, transferrin, and AlbuMAX™ I lipid-rich bovine serum albumin to enhance cell viability and function under reduced serum conditions.

Key Features & Benefits

✅ Serum-Reduced Formulation – Reduces FBS supplementation by 50-90% while maintaining optimal cell growth

✅ Enhanced Cell Growth & Viability – Contains trace elements and growth-promoting factors

✅ No Adaptation Required for Many Cell Lines – Supports Vero, MDCK, WI-38, PK-15, and Hep-2 cells without modification

✅ Sterile & Ready-to-Use – Sterile-filtered to ensure low contamination risk

✅ Customizable Formulation – Offers flexibility for specialized research needs

✅ Manufactured Under cGMP & ISO 13485 Standards – Ensures consistent performance and quality

Media Formulation

Contains:

✔ Glucose

✔ Non-Essential Amino Acids (NEAA)

✔ Sodium Pyruvate

✔ Phenol Red (pH Indicator)

Does Not Contain:

✖ L-glutamine (requires supplementation with 4 mM GlutaMAX™ or L-glutamine)

✖ HEPES Buffer

Technical Specifications

Recommended Supplementation & Cell Culture Guidelines

1️⃣ Supplement with FBS (1-5%) – Optimize concentration based on specific cell line requirements.

2️⃣ Add 4 mM L-glutamine or GlutaMAX™ – Required for optimal cell growth and metabolism.

3️⃣ Incubate at 37°C in 5-10% CO₂ – Maintains physiological pH due to the sodium bicarbonate buffer system (3.7 g/L).

4️⃣ Optimize FBS Levels Per Cell Line – Some cell lines may require different serum concentrations for optimal growth.

Applications

🔬 Mammalian Cell Culture & Expansion – Supports proliferation & maintenance of diverse cell lines

🧪 Biopharmaceutical & Cell Therapy Research – Used in drug development & cellular response studies

🦠 Virus Propagation & Vaccine Production – Suitable for Vero, MDCK, and WI-38 cells for viral research

⚗️ Recombinant Protein & Monoclonal Antibody Production – Reduces costs associated with serum supplementation

🏥 Stem Cell & Tissue Engineering Studies – Provides nutrient-rich conditions for advanced research applications

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for human or animal diagnostic or therapeutic applications.

⚠️ Use Standard Cell Culture Safety Precautions – Work in a biosafety cabinet (BSC) to prevent contamination.

⚠️ Store at 2–8°C & Protect from Light – Maintain media integrity and performance.

Why Choose Gibco™ Advanced MEM?

🔹 Reduces FBS Usage by 50-90% Without Affecting Cell Growth

🔹 Enhanced Formulation with Trace Elements & Growth Factors

🔹 Sterile, Ready-to-Use, and cGMP-Compliant

🔹 Validated for a Wide Range of Mammalian Cell Lines

🔹 Trusted Gibco™ Quality for Reliable Research Results

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀