Gibco™ McCoy's 5A (Modified) Medium

Gibco™ McCoy's 5A (Modified) is a general-purpose liquid medium designed to support the propagation of a wide range of mammalian primary cells, established cell lines, and biopsy explants. The medium's unique formulation ensures optimal cell growth for various applications, including tissue culture and research in molecular biology, immunology, and cell physiology.

Key Features:

1. Wide Cell Type Support:
   • Suitable for primary mammalian cells derived from bone marrow, skin, spleen, kidney, lung, rat embryos, and other tissues.
   • Supports both primary cells and established cell lines.
2. Modified Formulation:
   • With: High glucose, L-glutamine, bacto-peptone, phenol red, and sodium bicarbonate.
   • Without: Sodium pyruvate, HEPES buffer.
3. Buffering System:
   • Uses a sodium bicarbonate buffer system (2.2 g/L), requiring a 5–10% CO2 environment to maintain physiological pH.
4. Serum Supplementation:
   • Requires supplementation, commonly with 10% Fetal Bovine Serum (FBS), for optimal growth.
5. Animal Origin-Free:
   • Manufactured under cGMP compliance and certified to ISO 13485 standards, ensuring high quality and traceability.
6. Dr. McCoy's Proven Formula:
   • Includes reducing agents such as glutathione, and Hanks' salts for improved cell growth in non-CO2 incubators.

Specifications:

Applications:

1. Cell Propagation:
   • Ideal for culturing primary cells and established cell lines in mammalian cell research.
2. Tissue Explant Cultures:
   • Supports explants from biopsy tissues for histological and molecular studies.
3. Molecular Biology Research:
   • Suitable for use in molecular biology experiments such as gene expression, signal transduction, and transfection studies.
4. Immunology and Cytokine Research:
   • Provides optimal conditions for studies on immune cells and cytokine production.
5. Protein Expression:
   • Enhances the growth of cells used in recombinant protein production.

Advantages:

1. Proven Formula:
   • Based on Dr. McCoy's formulation with enhancements for modern research requirements.
2. Wide Cell Type Compatibility:
   • Supports a range of mammalian cells, including primary and established lines.
3. Flexibility in Incubation:
   • The addition of Hanks' salts enables use outside CO2 incubators, making it versatile for various lab setups.
4. Animal Origin-Free Certification:
   • Manufactured to reduce the risk of contamination from animal-derived materials, ideal for regulated research and manufacturing workflows.
5. Room-Temperature Shipping:
   • Simplifies logistics without compromising quality.

Protocol Overview:

1. Medium Preparation:
   • Supplement with 10% FBS or other serum according to experimental needs.
2. Adjust Culture Environment:
   • Maintain cells in a 5–10% CO2 incubator for proper pH buffering.
3. Cell Passaging:
   • Use standard cell culture techniques to passage cells regularly and maintain exponential growth.
4. Storage:
   • Store unused medium at 2–8°C, protected from light to prevent degradation.

Ordering Information:

Storage and Handling:

• Storage: 2–8°C, away from light.
• Shelf Life: 12 months.
• Shipping: Delivered at ambient temperature.

Related Products:

1. Gibco™ Fetal Bovine Serum (FBS): Recommended supplement for McCoy's 5A medium.
2. Gibco™ TrypLE™ Express: For gentle cell dissociation during passaging.
3. Gibco™ DMEM/F-12 Medium: For alternative culturing requirements.

Regulatory Notes:

• For Research Use Only: Not intended for human or animal diagnostic or therapeutic applications.

The Gibco™ McCoy's 5A (Modified) Medium is a versatile, high-quality solution for supporting a variety of mammalian cell types and experimental workflows. Its proven formula and flexible compatibility make it a reliable choice for research and manufacturing settings.