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Gibco™ Human M-CSF Recombinant Protein, PeproTech®
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Specifications:
| Application | Western Blot, ELISA-Based Assays, Functional Assay (FN) | ||
| Storage Temperature | -20°C | ||
| Product Type | Proteins & Peptides | Forms | lyophilized powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Gibco™ Human M-CSF (Macrophage Colony-Stimulating Factor) Recombinant Protein (PeproTech®) is a high-purity cytokine essential for the proliferation, differentiation, and survival of monocytes, macrophages, and osteoclast progenitor cells. Expressed in E. coli, this recombinant protein provides consistent bioactivity for immune cell research, stem cell differentiation, and oncology studies.
M-CSF plays a vital role in immune modulation, angiogenesis, and hematopoietic stem cell expansion, making it a key component in macrophage activation, osteoclast development, and inflammatory response research.
Key Features & Benefits
- Highly Purified & Bioactive
- Purity ≥98% by SDS-PAGE and HPLC, ensuring minimal contaminants.
- ED50 ≤ 1 ng/mL, promoting dose-dependent proliferation of murine M-NSF-60 cells.
- E. coli-Expressed for High Yield & Stability
- Provides consistent activity across various assays.
- Ideal for hematopoietic stem cell (HSC) expansion and immune modulation studies.
- Low Endotoxin for Reliable Experimental Results
- Endotoxin concentration <1 EU/µg, minimizing unwanted immune activation.
- Suitable for macrophage differentiation and cytokine research.
- Lyophilized for Long-Term Storage & Easy Reconstitution
- Easily reconstituted in sterile PBS or cell culture medium.
- Enables stable storage at -20°C to -80°C in working aliquots to prevent degradation.
- Optimized for Functional Assays & Cell Culture Studies
- Stimulates hematopoietic stem cells and myeloid differentiation.
- Supports colony formation and immune response regulation.
Product Specifications
| Feature | Details |
|---|---|
| Product Name | Gibco™ Human M-CSF Recombinant Protein (PeproTech®) |
| Catalog Number | 300-25-2UG |
| Expression System | E. coli |
| Amino Acid Sequence | Full-length human M-CSF (159 amino acids) |
| Molecular Weight | 36.8 kDa |
| Protein Class | Recombinant Cytokine |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Level | <1 EU/µg |
| Specific Activity | ED50 ≤ 1 ng/mL (determined via M-NSF-60 cell proliferation assay) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Storage Buffer | None (No Preservatives) |
| Storage Conditions | Lyophilized: -20°C, Reconstituted: -20°C to -80°C |
| Shipping Conditions | Ambient |
| Research Applications | Immunology, Oncology, Stem Cell Expansion, Osteoclast Differentiation |
Use Cases & Applications
1. Immunology & Macrophage Differentiation
- Monocyte-to-Macrophage Differentiation
- Stimulates monocyte proliferation and differentiation into macrophages.
- Enhances immune response and antigen presentation in immunological studies.
- Macrophage Activation & Functional Assays
- Induces VEGF secretion, supporting neovascularization.
- Used in macrophage-mediated tumor immunity and inflammation research.
2. Stem Cell Research & Osteoclast Formation
- Hematopoietic Stem Cell (HSC) Differentiation
- Supports differentiation of HSCs into monocyte-macrophage lineages.
- Used in stem cell expansion protocols.
- Osteoclast Progenitor Differentiation
- Promotes osteoclast development and bone resorption studies.
- Used in bone metabolism and osteoporosis research.
3. Cancer Research & Tumor Microenvironment Studies
- Role in Tumor-Associated Macrophages (TAMs)
- Supports studies on tumor progression and macrophage recruitment.
- Facilitates tumor immunosuppression modeling.
- VEGF-Mediated Angiogenesis Research
- Enhances endothelial cell mobilization and neovascularization.
- Used in anti-angiogenic drug screening.
4. Drug Discovery & High-Throughput Screening
- Cytokine-Based Drug Screening Assays
- Used in high-throughput screening of anti-inflammatory and immune-modulating drugs.
- Helps evaluate therapeutic compounds affecting macrophage differentiation.
- Biomarker Discovery & Immune Response Profiling
- Used for cytokine profiling in immune system disorders.
- Supports research on autoimmune diseases and chronic inflammatory conditions.
Recommended Reconstitution & Handling
Reconstitution Protocol:
- Centrifuge vial briefly before opening to bring lyophilized contents to the bottom.
- Reconstitute human M-CSF in sterile PBS or cell culture media to a concentration of 0.1–1.0 mg/mL.
- Aliquot & Store at -20°C to -80°C for extended stability.
- Avoid repeated freeze-thaw cycles to maintain protein activity.
Recommended Working Concentrations:
| Application | Concentration |
|---|---|
| Macrophage Differentiation | 10–50 ng/mL |
| Monocyte Proliferation | 5–25 ng/mL |
| Osteoclast Differentiation | 25–100 ng/mL |
| VEGF-Induced Angiogenesis Studies | 50–200 ng/mL |
Storage & Handling Instructions
- Lyophilized Storage: Store at -20°C in a desiccated environment.
- Reconstituted Storage: Store at ≤-20°C to -80°C in working aliquots.
- Avoid repeated freeze-thaw cycles to prevent degradation.
- Use aseptic techniques when handling and diluting the protein.
Why Choose Gibco™ Human M-CSF Recombinant Protein?
✅ High bioactivity with ED50 ≤ 1 ng/mL for macrophage proliferation
✅ E. coli-expressed for superior yield and stability
✅ Low endotoxin levels (<1 EU/µg) for reproducible cell culture studies
✅ Validated in functional assays including monocyte differentiation and VEGF secretion
✅ Versatile for immunology, stem cell, cancer, and drug discovery research
Gibco™ Human M-CSF Recombinant Protein is an essential cytokine for macrophage differentiation, immune modulation, and stem cell research, ensuring high-performance results in functional and cell-based assays.
- Pack Size: 1mg 50 µg 500 µg 100 µg 10 µg 250 µg 2 µg