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Gibco™ Human IL-3 Recombinant Protein, PeproTech®
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Specifications:
| Application | Western Blot, ELISA-Based Assays, Functional Assay (FN) | ||
| Storage Temperature | -20°C | ||
| Product Type | Proteins & Peptides | Forms | lyophilized powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Gibco™ Human IL-3 Recombinant Protein (PeproTech®) is a high-purity cytokine essential for hematopoietic cell proliferation, differentiation, and survival. Interleukin-3 (IL-3) is a key multipotent growth factor that regulates the development of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell, and monocytic lineages.
Produced in E. coli, this recombinant IL-3 is highly bioactive, with an ED50 ≤ 0.1 ng/mL, making it suitable for applications in stem cell research, immune cell activation, leukemia studies, and cytokine signaling research.
Key Features & Benefits
- Highly Purified & Bioactive
- Purity ≥98% by SDS-PAGE and HPLC, ensuring minimal contaminants.
- ED50 ≤ 0.1 ng/mL, promoting dose-dependent proliferation of human TF-1 cells.
- E. coli-Expressed for High Yield & Stability
- Provides consistent activity across various assays.
- Ideal for hematopoietic stem cell (HSC) expansion and immune modulation studies.
- Low Endotoxin for Reliable Experimental Results
- Endotoxin concentration <1 EU/µg, minimizing unwanted immune activation.
- Suitable for T-cell and dendritic cell culture experiments.
- Lyophilized for Long-Term Storage & Easy Reconstitution
- Easily reconstituted in sterile PBS or cell culture media.
- Enables stable storage at -20°C to -80°C in aliquots to prevent degradation.
- Optimized for Functional Assays & Cell Culture Studies
- Stimulates hematopoietic progenitor cells and myeloid differentiation.
- Supports colony formation and immune response regulation.
Product Specifications
| Feature | Details |
|---|---|
| Product Name | Gibco™ Human IL-3 Recombinant Protein (PeproTech®) |
| Catalog Number | 200-03-2UG |
| Expression System | E. coli |
| Amino Acid Sequence | Full-length human IL-3 (133 amino acids) |
| Molecular Weight | 15 kDa |
| Protein Class | Recombinant Cytokine |
| Purity | ≥98% by SDS-PAGE and HPLC |
| Endotoxin Level | <1 EU/µg |
| Specific Activity | ED50 ≤ 0.1 ng/mL (determined via TF-1 cell proliferation assay) |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Storage Buffer | None (No Preservatives) |
| Storage Conditions | Lyophilized: -20°C, Reconstituted: -20°C to -80°C |
| Shipping Conditions | Ambient |
| Research Applications | Hematopoiesis, Leukemia Research, Immunology, Stem Cell Expansion |
Use Cases & Applications
1. Hematopoiesis & Myeloid Differentiation
- Supports the Proliferation & Differentiation of Hematopoietic Cells
- Stimulates erythroid, megakaryocyte, neutrophil, and monocytic lineages.
- Used in bone marrow cell culture for stem cell research.
- Enhances Hematopoietic Stem Cell (HSC) Expansion
- Promotes multipotent colony formation in hematopoietic assays.
- Works synergistically with IL-6 and GM-CSF for immune modulation.
2. Immunology & Cytokine Research
- IL-3 in Mast Cell & Basophil Activation
- Regulates mast cell development and degranulation.
- Enhances allergic response studies and inflammatory signaling research.
- T-Cell & Dendritic Cell Proliferation
- Supports T-cell activation and cytokine secretion.
- Used in in vitro immune response modeling.
3. Leukemia & Cancer Research
- Role in Leukemia Cell Proliferation & Drug Resistance
- IL-3 influences tumor microenvironment interactions.
- Used in leukemia cell line maintenance and differentiation assays.
- Cancer Immunotherapy & Stem Cell Therapy Research
- Evaluates immune-modulating therapies targeting IL-3 pathways.
- Helps assess potential drug candidates affecting hematopoiesis.
4. High-Throughput Screening & Drug Discovery
- Screening of Hematopoietic Growth Factor Inhibitors
- Used in high-throughput assays for small molecule screening.
- Helps evaluate drug candidates affecting IL-3 signaling.
- Biomarker Discovery & Personalized Medicine
- Assists in cytokine profiling for hematologic disorders.
- Supports research in autoimmune and inflammatory diseases.
Recommended Reconstitution & Handling
Reconstitution Protocol:
- Centrifuge vial briefly before opening to bring lyophilized contents to the bottom.
- Reconstitute human IL-3 in sterile PBS or cell culture media to a concentration of 0.1–1.0 mg/mL.
- Aliquot & Store at -20°C to -80°C for extended stability.
- Avoid repeated freeze-thaw cycles to maintain protein activity.
Recommended Working Concentrations:
| Application | Concentration |
|---|---|
| Hematopoietic Stem Cell Expansion | 1–10 ng/mL |
| Leukemia Cell Proliferation Assays | 25–100 ng/mL |
| T-Cell & Mast Cell Activation | 5–50 ng/mL |
| Colony Formation Assays | 0.1–10 ng/mL |
Storage & Handling Instructions
- Lyophilized Storage: Store at -20°C in a desiccated environment.
- Reconstituted Storage: Store at ≤-20°C to -80°C in working aliquots.
- Avoid repeated freeze-thaw cycles to prevent degradation.
- Use aseptic techniques when handling and diluting the protein.
Why Choose Gibco™ Human IL-3 Recombinant Protein?
✅ High bioactivity (ED50 ≤ 0.1 ng/mL) for hematopoietic stem cell research
✅ E. coli-expressed for maximum yield and experimental consistency
✅ Low endotoxin levels (<1 EU/µg) for reliable immune studies
✅ Validated in functional assays for myeloid differentiation and leukemic cell proliferation
✅ Versatile for immunology, oncology, and hematopoietic research
Gibco™ Human IL-3 Recombinant Protein is a critical growth factor for hematopoiesis, immune cell regulation, and leukemia research, ensuring high-performance results in functional assays and cell-based experiments.
- Pack Size: 1mg 50 µg 500 µg 100 µg 10 µg 250 µg 2 µg