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Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-30°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Cell Culture | ||
Gibco™ Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution widely used in cell culture applications, such as washing cells, preparing reagents, and transporting tissue or cell samples. This formulation, specifically without calcium or magnesium, is ideal for rinsing chelators like EDTA from cultures before cell dissociation.
Key Features:
- Formulation: Free of calcium, magnesium, and phenol red.
- Applications: Washing cells before dissociation, transporting cells or tissues, diluting cells for counting, and reagent preparation.
- Sterile: Sterile-filtered for use in sensitive cell culture applications.
- Compliance: Manufactured under cGMP and ISO 13485 standards.
Specifications
| Attribute | Details |
|---|---|
| Product Type | DPBS (Dulbecco's Phosphate-Buffered Saline) |
| Formulation Additives | Without calcium, magnesium, and phenol red |
| Classification | Animal origin-free |
| Form | Liquid |
| Sterility | Sterile-filtered |
| Osmolality | 270–300 mOsm/kg |
| pH Range | 7.0–7.3 |
| Phenol Red Indicator | None |
| Dilution | 1X ready-to-use |
| Color | Clear |
| Shelf Life | 36 months from the date of manufacture |
| Storage Conditions | 15°C to 30°C |
| Shipping Conditions | Room temperature |
| Green Features | Sustainable packaging |
| Manufacturing Standards | cGMP-compliant under ISO 13485 |
Applications
- Cell Washing: Ideal for removing chelators (e.g., EDTA) prior to cell dissociation.
- Transport Medium: Safe for carrying cells or tissue samples.
- Dilution: Useful for preparing cells for counting or mixing reagents.
- Reagent Preparation: Compatible with most cell culture workflows.
Catalog Options
| Catalog Number | Quantity | Additional Information |
|---|---|---|
| 14190144 | 500 mL | Common size for routine lab use. |
| 14190086 | 100 mL | Compact size for minimal applications. |
| 14190185 | 20 x 100 mL | Pre-aliquoted for convenience. |
| 14190094 | 500 mL | Same size with alternate catalog name. |
| 14190250 | 10 x 500 mL | Bulk package for large-scale use. |
| 14190136 | 1000 mL | Larger size for high-usage labs. |
| 14190342 | 5 L | Suitable for industrial applications. |
| 14190359 | 10 L | For large-scale cell culture setups. |
| 14190240 | 10 L | Alternate catalog name for 10 L size. |
| 14190367 | 20 L | Maximum volume for extensive workflows. |
Advantages
- No Calcium or Magnesium: Prevents cell adhesion or aggregation, making it ideal for dissociation and EDTA rinsing workflows.
- Sterile and Clear: Ensures contaminant-free operations and ease of visual inspection.
- Sustainable Packaging: Eco-friendly design supports green initiatives.
- Room Temperature Shipping: Simplifies logistics without compromising product integrity.
Comparison with Other DPBS Products
| Feature | This Product (No Calcium/Magnesium) | Standard DPBS (with Calcium/Magnesium) |
|---|---|---|
| Use Case | Washing before dissociation | General cell maintenance and transport |
| Chelation | Compatible with chelators (e.g., EDTA) | Not suitable with chelators |
| Cell Behavior | Prevents adhesion/aggregation | Maintains normal cell adhesion properties |
Storage and Handling
- Storage Temperature: 15°C to 30°C.
- Shelf Life: 36 months, ensuring long-term usability.
- Shipping Condition: Room temperature to simplify handling during transit.
- Pack Size: 5 Liters 10 Liters 100 mL 500 mL 20 Liters 1000 mL 20 x 100 mL 10 x 500 mL
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