Gibco™ DMEM, powder, high glucose, pyruvate

Gibco™ DMEM (Dulbecco's Modified Eagle Medium) is a high-performance basal medium for culturing mammalian cells. The formulation, enriched with high glucose and sodium pyruvate, supports robust cell growth and viability, particularly for applications requiring high energy metabolism.

Key Features

1. Enhanced Nutrient Composition:
   • Contains 4x amino acids and vitamins compared to Eagle's MEM.
   • Supports rapid cell growth in demanding culture conditions.
2. With Additives:
   • High Glucose (4.5 g/L): Provides optimal energy source for metabolically active cells.
   • L-Glutamine: Enhances protein synthesis and cell metabolism.
   • Sodium Pyruvate: Functions as an additional energy source and precursor for biosynthetic pathways.
   • Phenol Red: Acts as a pH indicator.
3. Without Additives:
   • HEPES: Not included, requiring CO₂ buffering (5–10%).
   • Sodium Bicarbonate: To be supplemented during preparation.
4. Animal Origin-Free:
   • Compliant with ISO 13485 and cGMP standards for research and manufacturing consistency.
5. Powder Form:
   • Requires supplementation with sodium bicarbonate, pH adjustment, and filtration for final preparation.

Applications

• Cell Culture:
  • Ideal for culturing a variety of primary cells (fibroblasts, neurons, glial cells, HUVECs, smooth muscle cells).
  • Supports cell lines such as HeLa, 293, Cos-7, and PC-12.
• Protein Expression:
  • Suitable for recombinant protein production in transfected cell lines.
• Drug Discovery:
  • Used in high-throughput screening for cellular assays.
• Stem Cell Research:
  • Provides consistent growth support for stem cells and precursor cells.

Preparation Instructions

1. Suspend Powder:
   • Mix DMEM powder with distilled water.
   • Add sodium bicarbonate as per protocol.
2. Adjust pH:
   • Titrate to physiological pH (7.2–7.4).
3. Sterilize:
   • Filter through a 0.22 μm filter to ensure sterility.
4. Supplementation:
   • Add Fetal Bovine Serum (FBS) at a concentration of 10% for standard cell culture.
5. Storage:
   • Store prepared media at 2–8°C and protect from light.

Catalog-Specific Details

Advantages of Sodium Pyruvate

• Acts as an alternate energy source in glycolysis.
• Promotes cell survival under stress conditions.
• Enhances growth in cells with compromised metabolic pathways.

Specifications

• Form: Powder
• Shelf Life: 36 months from the date of manufacture.
• Storage: 2–8°C
• Shipping: Ambient conditions
• Sterility: Sterile-filtered
• Compliance: ISO 13485 and cGMP standards.

This DMEM variant, with high glucose and sodium pyruvate, is optimized for high-energy applications and supports reliable and reproducible cell culture results in a wide variety of research applications.