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Gibco™ Advanced DMEM, Sterile-filtered, High Glucose
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With: High Glucose, Non-essential Amino Acids, Sodium Pyruvate, Phenol Red
Without: L-glutamine
Specifications:
| Application | Cell culture | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | ||
Reduced-Serum, High-Performance Medium for Enhanced Mammalian Cell Culture Efficiency
Gibco™ Advanced DMEM (Dulbecco’s Modified Eagle Medium) is a high-performance basal medium formulated to support mammalian cell culture with reduced serum supplementation. Designed to deliver the same growth performance as classical DMEM, Advanced DMEM allows for a 50–90% reduction in Fetal Bovine Serum (FBS) usage—minimizing cost while maintaining optimal cell health, morphology, and proliferation.
Formulated with additional nutrients, lipid-rich AlbuMAX™ I, and trace elements, Advanced DMEM supports enhanced metabolic activity and reduced adaptation time for most cell lines. It is ideal for applications in biological research, protein production, drug screening, and high-throughput cell expansion.
Key Features & Benefits
-
Reduced Serum Requirements
Supports mammalian cell growth with only 1–5% FBS, offering up to 90% serum reduction without compromising cell performance. -
Enhanced Nutritional Profile
Includes ethanolamine, glutathione, ascorbic acid, insulin, transferrin, AlbuMAX™ I, and trace elements for optimal cell health. -
Ready for Diverse Cell Types
Proven to support MDBK, HepG2, COS-7, A549, MDCK, WI-38, and Vero cells without adaptation. -
Sterile & cGMP-Compliant
Manufactured under ISO 13485-certified, cGMP-compliant conditions for consistency and reproducibility. -
Stable & Convenient
Room temperature shipping and 12-month shelf life with 2–8°C storage; easy to incorporate into automated workflows.
Formulation Summary
| With Additives | Without Additives |
|---|---|
| High Glucose (4.5 g/L) | No Glutamine |
| Phenol Red | No HEPES |
| Sodium Pyruvate | |
| Non-Essential Amino Acids (NEAA) |
Note: Supplement with L-glutamine or GlutaMAX™ prior to use.
Applications
- Routine and large-scale mammalian cell culture
- Cell-based assay development
- Protein and antibody expression
- Vaccine development and viral production
- Biopharmaceutical research and high-throughput screening
- Studies requiring reduced animal serum exposure
Specifications Table
| Attribute | Details |
|---|---|
| Product Name | Advanced DMEM |
| Catalog Numbers | 12491015 (500 mL), 12491023 (10 × 500 mL) |
| Form | Liquid |
| Concentration | 1X |
| Sterility | Sterile-filtered |
| Buffer System | Sodium bicarbonate (3.7 g/L) |
| pH Stability | Requires 5–10% CO₂ environment |
| Serum Requirement | Reduced (1–5% FBS) |
| Storage Conditions | 2–8°C; protect from light |
| Shipping Conditions | Ambient |
| Shelf Life | 12 months from date of manufacture |
| Manufacturing Quality | cGMP-compliant under ISO 13485 |
| Intended Use | For Research Use Only |
Usage Guidelines
- Glutamine Supplementation: Add 4 mM L-glutamine or equivalent GlutaMAX™ to support optimal performance.
- Serum Optimization: Start with 1–5% FBS and adjust based on specific cell line requirements.
- Incubation Environment: Use in 5–10% CO₂ incubator to maintain physiological pH.
Gibco™ Advanced DMEM is a trusted, serum-reducing alternative to traditional DMEM. With its nutrient-enriched formulation and wide cell line compatibility, it enables cost-effective, high-quality cell culture for both research and bioproduction environments—without sacrificing cell performance.
- Pack Size: 500 mL 10 x 500 mL