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Thermo Scientific™ FastDigest™ Acc65I, 100 μL (100 Reactions)
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest Acc65I is a rapid restriction enzyme that recognizes the sequence 5′ G^GTACC 3′ / 3′ CCATG^G 5′, generating 5′ protruding ends. Optimized for use at 37 °C, it ensures complete DNA digestion in 5–15 minutes when used with the universal FastDigest Buffer system.
Part of the FastDigest™ enzyme portfolio, Acc65I is fully active in both FastDigest and FastDigest Green Buffers, eliminating the need for buffer changes in single, double, or multiple digestions. Its formulation minimizes star activity and supports direct downstream enzymatic reactions such as ligation, fill-in, or dephosphorylation.
Key Features
- Fast digestion – complete cleavage in 5–15 minutes.
- Universal buffer system – all FastDigest enzymes are fully active in one buffer.
- Convenient direct loading – FastDigest Green Buffer includes dyes and density reagent for electrophoresis.
- No star activity – optimized buffer ensures high-fidelity digestion.
- Downstream compatibility – T4 DNA Ligase, Klenow, T4 DNA Polymerase, and alkaline phosphatases work directly in the same buffer.
- Comprehensive portfolio – part of a range of 176 FastDigest enzymes.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping.
- SNP analysis.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | Acc65I |
| Recognition Sequence | 5′ G^GTACC 3′ / 3′ CCATG^G 5′ |
| Cleavage Product | 5′ protruding ends |
| Isoschizomers | Asp718I |
| Optimal Reaction Temperature | 37 °C |
| Digestion Time | 5–15 minutes |
| Compatible Buffers | 10X FastDigest Buffer, 10X FastDigest Green Buffer |
| Inactivation Temperature | 65 °C |
| Methylation Sensitivity | CpG methylation-sensitive; not Dam/Dcm-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Format
| Catalog Number | Quantity | Reactions | Format |
|---|---|---|---|
| FD0904 | 100 µL | ~100 reactions | Enzyme + Buffers |
Contents:
- FastDigest Acc65I enzyme (100 µL)
- 10X FastDigest Buffer (1.0 mL)
- 10X FastDigest Green Buffer (1.0 mL)
Thermo Scientific™ FastDigest Acc65I provides speed, convenience, and reliability in molecular cloning workflows. Its universal buffer system allows rapid digestion and seamless downstream compatibility, while optimized conditions prevent star activity. Delivering complete digestion in minutes, Acc65I is an ideal choice for researchers requiring high-performance restriction digestion with reproducible results.