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Thermo Scientific™ FastDigest™ Eco105I, 50 μL (50 Reactions)
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Thermo Scientific™ FastDigest Eco105I is a high-performance restriction enzyme that recognizes the sequence 5′ TAC^GTA 3′ / 3′ ATG^CAT 5′, generating blunt ends. It is optimized for rapid DNA digestion, achieving complete cleavage within 5–15 minutes at 37 °C using the universal FastDigest Buffer system.
As part of the FastDigest™ enzyme portfolio, Eco105I is fully active in both FastDigest and FastDigest Green Buffers, ensuring maximum convenience for single, double, or multiple digestions without buffer changes or DNA clean-up steps. Its optimized buffer composition prevents star activity and is fully compatible with downstream DNA-modifying enzymes such as T4 DNA Ligase, Klenow Fragment, T4 DNA Polymerase, and alkaline phosphatases.
Key Features
- Fast performance – complete DNA digestion in 5–15 minutes at 37 °C.
- Universal buffer system – 100% activity of all FastDigest enzymes in a single buffer.
- Direct gel loading – FastDigest Green Buffer includes density reagent and tracking dyes.
- No star activity – optimized formulation eliminates nonspecific cleavage.
- Streamlined workflows – downstream enzymatic applications proceed without additional clean-up.
- Wide selection – one of 176 enzymes available in the FastDigest product line.
Applications
- Molecular cloning.
- Restriction site mapping.
- Genotyping.
- Southern blotting.
- Restriction fragment length polymorphism (RFLP).
- SNP analysis.
Technical Specifications
| Property | Value |
|---|---|
| Enzyme | Eco105I |
| Recognition Sequence | 5′ TAC^GTA 3′ / 3′ ATG^CAT 5′ |
| Cleavage Product | Blunt ends |
| Isoschizomers | SnaBI, BstSNI |
| Optimal Reaction Temperature | 37 °C |
| Digestion Time | 5–15 minutes |
| Compatible Buffers | 10X FastDigest Buffer, 10X FastDigest Green Buffer |
| Inactivation Temperature | 65 °C |
| Methylation Sensitivity | CpG methylation-sensitive; not Dam/Dcm-sensitive |
| Heat Inactivation | Yes |
| Type IIS Restriction Enzyme | No |
| Research Category | Traditional cloning |
| Storage Temperature | –20 °C |
Available Formats
| Catalog Number | Quantity | Reactions | Format |
|---|---|---|---|
| FD0404 | 50 µL | ~50 reactions | Enzyme + Buffers |
Contents:
- FastDigest Eco105I enzyme (50 µL)
- 10X FastDigest Buffer (1.0 mL)
- 10X FastDigest Green Buffer (1.0 mL)
Thermo Scientific™ FastDigest Eco105I delivers rapid, accurate, and high-fidelity restriction digestion with blunt-end cleavage. With its universal buffer system, compatibility with downstream enzymes, and optimized performance that prevents star activity, it streamlines molecular cloning and DNA analysis workflows. Eco105I is a reliable choice for researchers seeking speed, reproducibility, and flexibility in their molecular biology experiments.