Luminex® Human XL Cytokine Kit Performance Assay

Product Features

Magnetic Luminex High-PerformancHigh-Performance Assays are flexible bead-based multiplex assays. They allow up to 50 user-defined target analytes to be simultaneously profiled using cell culture supernates, serum, or plasma samples. Some panels are also validated with human milk, saliva, and/or urine samples.

• Simultaneously profile up to 46 analytes of your choice in one sample
• Magnetic format allows for easier wash steps
• Undergoes similar validation testing as Quantikine® ELISA Kits
• Precise and Specific
• Requires a small sample volume (<50 µL)
• Run your assay in just 3-5 hours
• Mass-calibrated standards for consistent results with every new lot of material
• Enough reagents for one 96-well plate

Kit Components

• Premixed cocktail of antibody-coated Magnetic beads
• Premixed cocktail of biotinylated detection antibody
• Standard Cocktail(s)
• Bead Diluent
• Biotin Antibody Diluent
• Calibrator Diluent
• Wash Buffer
• Streptavidin-PE
• One flat-bottom 96-well Microplate
• Foil Plate Sealers (4)
• Mixing Bottle
• Standard Value Card**
• High and low controls with Control Mean Value Card

**A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Kit.

Precision

Accuracy

Which Luminex Assay is right for me?

Assays for the Luminex platform are offered as High Performance Assays or Assays. The Luminex High Performance Assays are fully validated panels with a focused selection of analytes. They are available in We Mix, You Mix, and Predetermined formats. The Luminex Assays allows for the maximum number of analytes in a multiplex and is supplied as a premixed kit. View a table comparing the features and benefits of these bead-based multiplex assays.

More Information About Magnetic Bead-based Multiplex Kits For The Luminex® Platform

Magnetic Luminex High Performance Assays are designed for use with the Luminex MAGPIX CCD Imager. Alternatively, kits can be used with the Luminex 100/200™ or FLEXMAP 3D, dual laser, flow-based sorting and detection platforms.

Analyte-specific antibodies are pre-coated onto color-coded microparticles. Microparticles, standards, and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, Streptavidin-Phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated detection antibodies, is added to each well. A final wash removes unbound Streptavidin-PE and the microparticles are resuspended in buffer and read using the Luminex or Bio-Plex analyzer. One laser is bead-specific and determines which analyte is being detected. A magnet in the analyzer captures and holds the superparaMagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.