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Sigma-Aldrich Folic acid assay medium, suitable for microbiology, NutriSelect® Plus, 100g
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for the microbiological assay of folic acid using Streptococcus faecium as the test organism
Specifications:
| Application | Microbiology | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Sigma-Aldrich | ||
| Product Grade | Microbiology grade | ||
Sigma-Aldrich Folic Acid Assay Medium, part of the NutriSelect® Plus line, is a non-selective culture medium specifically formulated for the quantitative microbiological assay of folic acid using Streptococcus faecium as the test organism. This balanced medium supports robust microbial growth and precise nutrient-based assays in microbiology, food testing, and pharmaceutical analysis.
Key Features
- Optimized for Folic Acid Assay – Supports growth of Streptococcus faecium for vitamin B9 quantification
- NutriSelect® Plus Grade – High-quality medium suitable for analytical and industrial microbiology
- Defined Composition – Contains vitamin-free casein hydrolysate and micronutrients for controlled microbial growth
- Versatile Applications – Used in pharmaceutical, food, and microbiological laboratories
- Non-selective for Streptococcus spp. – Suitable for broad-spectrum folic acid bioassay development
Technical Specifications
| Property | Specification |
|---|---|
| Form | Powder |
| Sterility | Non-sterile |
| pH (at 25 °C) | 6.8 ± 0.2 |
| Storage Temperature | 2–8 °C |
| Shelf Life | Limited (see label for expiry date) |
| Suitability | Nonselective for Streptococcus spp. |
| Technique | Microbiological culture |
| Quality Level | 200 |
| Recommended Use | Microbiological assay of folic acid |
| Manufacturer/Tradename | NutriSelect® Plus |
| UNSPSC Code | 41171608 |
| NACRES Code | NA.85 |
Application Areas
- Pharmaceutical Quality Control – Vitamin B9 quantification in formulations
- Food and Beverage Testing – Nutritional labeling and fortification verification
- Microbiological Research – Nutrient-based microbial assay development
Components (per Liter)
| Component | Amount (g/L) |
|---|---|
| Casein Acid Hydrolysate, Vitamin Free | 12.00 |
| Dextrose | 40.00 |
| Sodium Citrate | 20.00 |
| L-Cystine | 0.20 |
| DL-Tryptophan | 0.20 |
| Adenine Sulfate | 0.02 |
| Guanine Hydrochloride | 0.02 |
| Uracil | 0.02 |
| Thiamine Hydrochloride | 0.002 |
| Pyridoxine Hydrochloride | 0.004 |
| Riboflavin (Vitamin B2) | 0.002 |
| Niacin | 0.002 |
| p-Aminobenzoic Acid (PABA) | 0.0002 |
| Biotin | 0.0000008 |
| Calcium Pantothenate | 0.0004 |
| Dipotassium Phosphate | 1.00 |
| Monopotassium Phosphate | 1.00 |
| Magnesium Sulfate | 0.40 |
| Sodium Chloride | 0.02 |
| Ferrous Sulfate | 0.02 |
| Manganese Sulfate | 0.02 |
Preparation Instructions
- Suspend 7.5 g of medium in 100 mL distilled water
- Boil to dissolve completely, mix to evenly distribute precipitate
- Dispense 5 mL per assay tube and add 5 mL distilled water
- Autoclave at 121 °C (15 lbs pressure) for 10 minutes
- Cool immediately before use
Ordering Information
| Catalog Number | Pack Size | Availability |
|---|---|---|
| F5422-100G | 100 g | In stock (UK) |
Sigma-Aldrich NutriSelect® Plus Folic Acid Assay Medium provides a reliable, nutrient-defined formulation for folic acid quantification assays in pharmaceutical and food testing. With optimized nutrients and consistent performance, it is a trusted solution for folate assay protocols involving Streptococcus faecium.