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Invitrogen™ FluoReporter™ lacZ Flow Cytometry Kit
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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Flow Cytometry Reagents | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Fluorescence-based β-galactosidase detection in single cells by flow cytometry
The Invitrogen™ FluoReporter™ lacZ Flow Cytometry Kit provides a complete set of reagents and protocols for quantitative analysis of β-galactosidase (lacZ) activity at the single-cell level using flow cytometry. This system utilizes fluorescein di-β-D-galactopyranoside (FDG), a non-fluorescent substrate that is cleaved by β-galactosidase to yield bright green-fluorescent fluorescein.
The kit includes a license to practice patented FDG loading methods involving hypotonic shock to improve substrate entry into cells and techniques to enhance fluorescein retention in lacZ-positive cells, ensuring sensitive and specific detection.
Key Features
- Single-Cell Resolution – Detect lacZ expression in individual cells by flow cytometry
- High Sensitivity – Hypotonic shock loading increases FDG uptake and detection signal
- Improved Signal Retention – Proprietary methods reduce loss of fluorescent product from lacZ-positive cells
- Complete Kit – Includes FDG substrate, β-galactosidase inhibitor PETG, chloroquine diphosphate, and propidium iodide
- Multiplex Capability – Propidium iodide enables viability discrimination during analysis
Applications
- lacZ reporter gene detection in mammalian cells
- Quantification of transfection or transduction efficiency
- Flow cytometry–based gene expression analysis
- Cell sorting based on lacZ activity and viability
- Functional assays for gene regulation studies
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | F1930 (also known as F-1930) |
| Assay Type | β-galactosidase (lacZ) activity assay |
| Detection Method | Fluorescence (FITC channel) |
| Substrate | Fluorescein di-β-D-galactopyranoside (FDG) |
| Kit Components | FDG (0.5 mL, 20 mM), PETG (2.0 mL, 50 mM), chloroquine diphosphate (1.0 mL, 30 mM), propidium iodide (1.0 mL, 150 μM) |
| Storage Conditions | –5°C to –30°C, protect from light |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
The FluoReporter™ lacZ Flow Cytometry Kit delivers a highly sensitive, quantitative, and single-cell–level solution for detecting lacZ reporter expression. Its optimized loading and retention methods make it ideal for flow cytometry-based reporter assays, enabling accurate discrimination between lacZ-positive and negative cell populations.