ONT Flow Cell Priming Kit for 6 Rxns

The Flow Cell Priming Kit is a vital reagent set designed to support sequencing experiments using Oxford Nanopore Technologies' V14 chemistry. This kit allows for priming of flow cells, including MinION, GridION, and PromethION devices, as well as reloading libraries during sequencing. It is compatible with most V14 kits and ensures optimal flow cell performance during experiments.

Key Features and Benefits

1. Compatibility:
   • Works with a wide range of V14 kits, including Ligation Sequencing Kit V14, Rapid Barcoding Kits, Native Barcoding Kits, and others.
   • Suitable for MinION Mk1B, Mk1C, GridION, and PromethION devices.
   • Supports R10.4.1 flow cells.
2. Ease of Use:
   • Pre-measured reagents for consistent priming and reloading.
   • Allows for multiple uses across six sequencing runs.
3. Sustainability:
   • Shipped in temperature-controlled, environmentally friendly packaging.
4. Storage:
   • Stable at 2–8°C during shipping.
   • Long-term storage at -20°C to -30°C for extended usability.
5. What’s Included:
   • Flow Cell Flush (FCF): 1 vial (8,000 µL).
   • Flow Cell Tether (FCT): 1 vial (200 µL).

Specifications

Applications

1. Flow Cell Priming:
   • Prepares MinION, GridION, and PromethION flow cells for sequencing.
2. Library Reloading:
   • Reloads additional libraries onto flow cells during ongoing experiments.
3. Optimized for V14 Chemistry:
   • Ensures compatibility with the latest sequencing kits and auxiliary kits.

Compatibility

Kits:

• Ligation Sequencing Kits (SQK-LSK114, SQK-LSK114-XL).
• Rapid Barcoding Kits (SQK-RBK114.24, SQK-RBK114.96).
• Native Barcoding Kits (SQK-NBD114.24, SQK-NBD114.96).
• cDNA and 16S Barcoding Kits.

Flow Cells:

• R10.4.1 (FLO-MIN114, FLO-PRO114M).

Devices:

• MinION Mk1B.
• MinION Mk1C.
• GridION.
• PromethION.

Usage Notes

• This kit is only compatible with V14 chemistry and cannot be used with other versions.
• Store reagents properly to maintain functionality over the long term.
• For Ultra-Long DNA Sequencing Kit V14, specific reagents are required.

Why Choose the Flow Cell Priming Kit?

• Ensures optimal performance of flow cells for consistent sequencing results.
• Streamlines the process of flow cell preparation and library reloading.
• Compatible with a wide range of V14 kits and devices for maximum flexibility.

This kit is essential for researchers utilizing Oxford Nanopore Technologies' V14 sequencing chemistry.