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Thermo Scientific™ Exonuclease III (200 U/μL) - 4,000 units
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Specifications:
| Application | Cloning | ||
| Storage Temperature | -20°C | ||
| Product Type | Enzymes and Substrates | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Exonuclease III (ExoIII) is a versatile enzyme with four catalytic activities, making it an essential tool in molecular biology. Its primary 3′→5′ exodeoxyribonuclease activity is specific for double-stranded DNA (dsDNA), enabling controlled degradation from blunt ends, 5′-overhangs, or nicks. This activity releases 5′-mononucleotides and generates long stretches of single-stranded DNA, useful for sequencing and mutagenesis applications.
In addition, ExoIII exhibits 3′-phosphatase activity, RNase H activity, and apurinic/apyrimidinic (AP) endonuclease activity, extending its applications to DNA repair studies, probe preparation, and hybrid digestion. Importantly, ExoIII is inactive on 3′-overhang ends longer than four bases, DNA with 3′-terminal C-residues, and phosphorothioate-linked nucleotides, ensuring reaction specificity.
Key Features
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Multi-functional Enzyme – Exhibits four catalytic activities:
- 3′→5′ exodeoxyribonuclease (dsDNA-specific)
- 3′-phosphatase activity
- RNase H activity (RNA-DNA hybrids)
- AP-endonuclease activity (cleaves at AP sites)
- Specificity – Inactive on long 3′-overhangs (>4 bases) and phosphorothioate linkages.
- Buffer Compatibility – Active in standard restriction enzyme buffers.
- High Purity – Tested free of contaminating endonucleases and exonucleases.
Applications
- Generation of unidirectional deletions in DNA fragments (with S1 nuclease)
- Preparation of single-stranded templates for dideoxy sequencing
- Site-directed mutagenesis workflows
- Cloning of PCR products
- Preparation of strand-specific probes
- DNA repair and mutation studies
Technical Specifications
| Property | Details |
|---|---|
| Product Type | Exonuclease III |
| Catalog Number | EN0191 |
| Quantity | 4,000 units |
| Concentration | 200 U/µL |
| Enzyme | Exonuclease III |
| Catalytic Activities | 3′→5′ exonuclease, 3′-phosphatase, RNase H, AP-endonuclease |
| Substrate Specificity | dsDNA (blunt ends, 5′-overhangs, nicks); RNA-DNA hybrids |
| Inactive On | 3′-overhangs ≥ 4 bases, 3′-terminal C-residues, phosphorothioate nucleotides |
| Compatible Buffer | Enzyme/Restriction enzyme buffers |
| Storage Conditions | –20 °C |
| Unit Size | Each |
Ordering Information
| Catalog Number | Quantity | Concentration | Unit Size |
|---|---|---|---|
| EN0191 | 4,000 units | 200 U/µL | Each |
The Thermo Scientific™ Exonuclease III (ExoIII) is a multi-functional DNA-processing enzyme ideal for applications in DNA sequencing, mutagenesis, cloning, and repair studies. With four catalytic activities, high specificity, and compatibility with standard reaction buffers, ExoIII is a powerful, reliable tool for advanced molecular biology and genetic engineering workflows.