ELISpot™ Plus: Human TNF-α (ALP) ELISA Kit

The Mabtech™ ELISpot™ Plus: Human TNF-α (ALP) ELISA Kit is specifically designed for the enumeration of human TNF-α-secreting cells using the ELISpot assay. This kit offers high specificity for Tumor Necrosis Factor-α (TNF-α) and is ideal for research into immune regulation, inflammation, and cytokine signaling.

Intended Use

• Purpose:
  • Enumerate cells secreting human TNF-α using the ELISpot assay.
• Usage:
  • For research use only. Not for use in diagnostic procedures.

Product Details

• Product Name: ELISpot Plus: Human TNF-α (ALP)
• Application: ELISpot
• Analyte: TNF-α
• Reactivity: Human
• Specificity:
  • Based on a matched pair of monoclonal antibodies (mAbs) specific for human TNF-α.

Shipping and Storage

• Shipping Conditions:
  • Shipped at ambient temperature.
• Storage Conditions:
  • Store antibodies, enzyme conjugate, and substrate at 4–8°C upon receipt.
  • Plates can be stored at room temperature.
• Shelf Life:
  • At least 18 months from the date of receipt.

Analyte Information: TNF-α

Tumor Necrosis Factor-α (TNF-α):

• Function:
  • Produced by monocytes, macrophages, T cells, and B cells.
  • Plays a critical role in immune system regulation, protection against bacterial infections, cell growth modulation, and involvement in septic shock.
• Cell Types Producing TNF-α:
  • T cells (Tc, Th1, Th2, Th17, Tfh)
  • Monocytes/Macrophages
• Clinical Importance:
  • Dysregulation of TNF-α is linked to autoimmune diseases, chronic inflammation, and septic shock.

Applications

• Immune Research:
  • Study TNF-α secretion in immune response assays.
• Inflammation Studies:
  • Assess TNF-α levels in inflammatory conditions and diseases.
• Cytokine Profiling:
  • Use in cytokine profiling assays to study immune system regulation.
• Infectious Disease Research:
  • Investigate TNF-α responses in bacterial and viral infections.
• Therapeutic Development:
  • Evaluate TNF-α-targeting drugs in autoimmune and inflammatory disease models.

Key Features

1. Matched mAbs:
   • High specificity for human TNF-α ensures accurate results.
2. Long Shelf Life:
   • Reliable for at least 18 months under proper storage conditions.
3. Ease of Storage:
   • Room temperature storage for plates simplifies laboratory logistics.
4. Cross-Applicability:
   • Suitable for studying both innate and adaptive immune responses.

Alternative Names for TNF-α

• Tumor necrosis factor-α
• TNF-α
• TNF-alpha
• TNFa
• DIF-alpha
• TNFSF2
• TNLG1F

The Mabtech™ ELISpot™ Plus: Human TNF-α (ALP) ELISA Kit is an essential tool for researchers studying immune responses, cytokine signaling, and inflammatory pathways. Its specificity, ease of use, and long shelf life make it a reliable choice for experimental and clinical research applications.