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Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit, 2 x 96 Tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Sensitive, species-independent colorimetric assay for total SOD activity measurement in biological samples
The Invitrogen™ Superoxide Dismutase (SOD) Colorimetric Activity Kit provides a complete, ready-to-use solution for quantifying total superoxide dismutase activity in serum, plasma, tissues, cells, and erythrocyte lysates. This species-independent assay measures the activity of all SOD isoforms, including Cu/Zn-SOD (SOD1), Mn-SOD (SOD2), and Fe-SOD.
Using a 96-well microplate format and a 450 nm colorimetric readout, the assay detects SOD activity as low as 0.044 U/mL, with a standard curve range of 1.4–92 U/mL. The kit is designed for ease of use, enabling high-throughput and reproducible results for oxidative stress and antioxidant research.
Key Features
- High Sensitivity – Detects SOD activity down to 0.044 U/mL
- Broad Sample Compatibility – Works with serum, plasma, cell lysates, tissues, and erythrocyte lysates
- Comprehensive Detection – Measures Cu/Zn-, Mn-, and Fe-SOD isoforms
- Ready-to-Use Format – Includes all reagents, standards, and plates
- Quantitative – Generates a standard curve using bovine erythrocyte SOD
- Species-Independent – Validated across multiple biological sources
Applications
- Total SOD activity quantification in biological and environmental samples
- Oxidative stress research and free radical biology
- Antioxidant profiling in disease and aging studies
- Cell and tissue physiology investigations involving reactive oxygen species
- Enzyme kinetics and inhibitor screening
Assay Principle
The kit measures SOD activity by detecting the inhibition of superoxide-mediated substrate oxidation.
- Xanthine oxidase generates superoxide in the presence of oxygen.
- Superoxide reacts with a colorless substrate in the detection reagent, producing a yellow product measured at 450 nm.
- SOD in the sample scavenges superoxide, reducing product formation. Higher SOD activity results in lower absorbance values.
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | EIASODC |
| Assay Type | Colorimetric enzyme activity assay |
| Detection Method | Absorbance at 450 nm |
| Sensitivity | 0.044 U/mL |
| Standard Curve Range | 1.4–92 U/mL |
| Assay Format | 2 × 96-well plates |
| Sample Types | Serum, plasma, cells, tissues, erythrocyte lysates |
| Storage Conditions | –20°C |
| Shipping Conditions | Wet or dry ice |
| Regulatory Status | Research Use Only (RUO) |
The Invitrogen™ SOD Colorimetric Activity Kit delivers accurate, sensitive, and reproducible measurement of superoxide dismutase activity for antioxidant and oxidative stress research. Its ready-to-use format, broad sample compatibility, and high sensitivity make it ideal for biomedical, pharmaceutical, and environmental studies.