Invitrogen™ Galactose Colorimetric Detection Kit, 2 x 96 Tests

The Invitrogen™ Galactose Colorimetric Detection Kit is a complete, ready-to-use assay for the quantitative measurement of galactose in serum, plasma, buffers, and tissue culture media. Utilizing a robust colorimetric format with detection at 560 nm, the kit provides high sensitivity (0.493 mg/dL) and a broad standard curve range (0.78–25 mg/dL) for accurate galactose quantification.

Assay Principle

The assay measures galactose based on galactose oxidase-mediated oxidation:

1. Galactose oxidase reacts with D-(+)-galactose to generate hydrogen peroxide (H₂O₂).
2. In the presence of horseradish peroxidase (HRP), H₂O₂ reacts with a proprietary substrate to produce a pink-colored product.
3. The absorbance is read at 560 nm using a microplate reader, with color intensity proportional to galactose concentration.

Key Features

• High Sensitivity – Detects as low as 0.493 mg/dL galactose.
• Broad Quantification Range – 0.78–25 mg/dL standard curve.
• Ready-to-Use Kit – Includes plates, standards, buffers, and all required reagents.
• Species Independent – Works across multiple biological sources.
• Simple Workflow – Room temperature 30-minute incubation with direct readout.

Applications

• Metabolic Studies – Assess galactose metabolism in research models.
• Food & Nutrition Research – Measure galactose in dairy, fruit, and vegetable samples (research use only).
• Enzyme Activity Studies – Monitor galactose oxidase-dependent reactions.
• Cell Culture Monitoring – Evaluate sugar metabolism in tissue culture systems.
• Biochemical Pathway Analysis – Study the Leloir pathway and related disorders.

Technical Specifications

Kit Contents

• 96-well microplates
• D-(+)-Galactose standard
• Assay buffer
• Galactose oxidase
• Horseradish peroxidase (HRP)
• Substrate solution
• Lot-specific technical datasheet

The Invitrogen™ Galactose Colorimetric Detection Kit is a reliable and precise solution for galactose measurement in biological and biochemical research applications. With its ready-to-use format, high sensitivity, and broad detection range, it is well-suited for metabolic research, enzyme assays, and nutritional studies.