1-Step™ TMB ELISA Substrate Solution

The 1-Step™ TMB ELISA Substrate Solutions by Thermo Scientific™ are ready-to-use, non-carcinogenic substrates for detecting horseradish peroxidase (HRP) activity in ELISA assays. These substrates yield a blue color upon oxidation by HRP, which shifts to yellow upon the addition of a stop solution (sulfuric or phosphoric acid).

Key Features:

1. Versatile Sensitivities:
   • Available in three formulations:
     • 1-Step Slow TMB: Intermediate sensitivity.
     • 1-Step Turbo TMB: High sensitivity, comparable to OPD at ~1 mg/mL.
     • 1-Step Ultra TMB-ELISA: Highest sensitivity with a signal-to-noise ratio in the picogram range.
2. Colorimetric Detection:
   • Initial blue color (Amax = 370 nm and 652 nm).
   • Converts to yellow upon stopping the reaction (Amax = 450 nm).
3. Enhanced Performance:
   • Faster oxidation than other HRP substrates.
   • Free of DMF (dimethylformamide) or DMSO (dimethyl sulfoxide).
4. User-Friendly:
   • Ready-to-use with no additional preparation or filtering.
   • Suitable for kinetic and endpoint ELISA assays.
5. Non-Carcinogenic:
   • Safer for laboratory use compared to traditional HRP substrates.

Specifications:

Product Variants:

Applications:

• Quantitative and qualitative ELISA.
• Kinetic or endpoint assays.
• Gene expression, immunology, and diagnostic research.

Advantages:

1. Custom Sensitivity Options: Choose the formulation best suited to your assay requirements.
2. Ready-to-Use: Simplifies workflows and minimizes preparation errors.
3. High Signal-to-Noise Ratio: Ensures reliable and reproducible results.
4. Safety: Non-carcinogenic and free of DMF/DMSO, making it safer for routine laboratory use.

Comparison of Sensitivity Levels:

Storage and Handling:

• Store at 4°C upon receipt.
• Protect from moisture and direct light for optimal stability.

References:

1. Josephy, P.D., et al. (1982). J. Biol. Chem. 257(7), 3669-3675.
2. Josephy, P.D., et al. (1983). J. Biol. Chem. 258(9), 5561-5569.
3. Liem, H.H., et al. (1979). Anal. Biochem. 98, 388-393.