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Invitrogen™ Creatinine Urinary Detection Kit, 2 x 96 Tests
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -20°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Creatinine Urinary Detection Kit is a reliable colorimetric assay designed for the quantitative determination of creatinine in urine samples from multiple species, including human, rat, dog, and monkey. This ready-to-use kit provides a simple and efficient solution for laboratories conducting renal function studies and metabolic research.
Based on a modified Jaffe reaction, the assay enables accurate detection of creatinine levels by measuring the intensity of a colorimetric signal at 490 nm, which is directly proportional to creatinine concentration. The kit includes all necessary reagents, standards, and plates for streamlined workflow and reproducible results.
Key Features
- Sensitive colorimetric detection with a limit of 0.019 mg/dL
- Broad quantification range (0.3–20 mg/dL)
- Compatible with multiple species urine samples
- Complete kit with all required reagents included
- Easy-to-follow protocol for rapid and reproducible results
- Optimized modified Jaffe reaction for urine analysis
- Suitable for high-throughput 96-well plate assays
Applications
- Renal function and kidney health studies
- Glomerular filtration rate (GFR) estimation (creatinine clearance)
- Metabolic and physiological research
- Drug toxicity and pharmacokinetic studies
- Biomarker analysis in preclinical and translational research
Assay Principle
The assay quantifies creatinine using a modified Jaffe reaction:
- Samples and standards are added to a 96-well microplate
- The creatinine detection reagent is added to initiate the reaction
- A colorimetric product forms in proportion to creatinine concentration
- Absorbance is measured at 490 nm (with optional correction at 570–590 nm)
- Sample concentrations are calculated using a standard calibration curve
Performance Characteristics
| Parameter | Details |
|---|---|
| Detection Method | Colorimetric |
| Sensitivity | 0.019 mg/dL |
| Assay Range | 0.3 – 20 mg/dL |
| Sample Type | Urine |
| Species Compatibility | Human, rat, dog, monkey |
| Plate Format | 96-well |
| Equipment Required | Microplate reader (490 nm) |
Kit Contents
- 96-well microplate(s)
- Creatinine standard
- Creatinine detection reagent
- Assay buffers and reagents
- Lot-specific technical data sheet
Product Variant
| Catalog Number | Quantity |
|---|---|
| EIACUN | 2 × 96 tests |
Storage & Handling
- Store at –20°C
- Ship under wet or dry ice conditions
- Avoid repeated freeze-thaw cycles for optimal performance
Important Notes
- Suitable for urine samples only
- Mouse urine samples are not compatible for GFR determination using this assay
- For research use only; not for diagnostic procedures
The Invitrogen™ Creatinine Urinary Detection Kit offers a robust and efficient solution for the quantitative analysis of creatinine in urine samples. Its high sensitivity, broad detection range, and user-friendly format make it ideal for laboratories conducting renal function studies, metabolic research, and biomarker analysis.