Invitrogen™ Human COX-2 ELISA Kit

The Invitrogen™ Human COX-2 ELISA Kit is a highly sensitive sandwich ELISA designed for the quantitative measurement of human cyclooxygenase-2 (COX-2) in various biological samples, including plasma, serum, and supernatants. It is optimized for research applications in cancer, inflammation, and other pathological studies involving COX-2.

Key Features:

• Analytical Sensitivity: 1.2 ng/mL.
• Assay Range: 1.2–300 ng/mL, enabling broad detection capabilities.
• Sample Types: Suitable for plasma, serum, and supernatant with a minimum volume of 50 µL.
• Assay Format: Sandwich ELISA for increased specificity.
• Time-to-Result: Total runtime is approximately 4 hours and 45 minutes, with hands-on preparation time of 1 hour and 20 minutes.
• Reproducibility:
  • Intraassay CV: <10%.
  • Interassay CV: <12%.
• Detection Method: Colorimetric readout using a microplate reader.
• Detector Antibody Conjugate: Biotinylated detection antibody with HRP (Horseradish Peroxidase) label.

Kit Contents:

1. Pre-coated 96-well plate.
2. Standard for calibration.
3. Assay diluent concentrate.
4. Biotinylated detection antibody.
5. SAV-HRP (streptavidin-HRP conjugate).
6. Wash buffer.
7. Chromogen substrate for color development.
8. Stop solution to terminate the reaction.
9. Adhesive plate covers to prevent contamination.

Storage and Shipping:

• Shipping Conditions: Wet ice.
• Storage Requirements: 2–8°C.

Applications:

1. Inflammatory Research: Measures COX-2 levels involved in inflammatory pathways and prostaglandin synthesis.
2. Cancer Studies: Detects COX-2 upregulation, which is associated with tumor growth, angiogenesis, and apoptosis resistance.
3. Drug Screening: Evaluates the impact of NSAIDs and other inhibitors targeting COX-2 activity.
4. Neuroscience: Investigates COX-2 expression in the brain and its involvement in neuroinflammation.

Background Information:

• COX-2 Function: Converts arachidonate into prostaglandin H2 (PGH2), a precursor for prostaglandins involved in inflammation and cancer progression.
• Expression Patterns:
  • Physiological: Endothelium, kidney, and brain.
  • Pathological: Upregulated in inflammation, cancer, and other disease conditions.
• Key Inhibitors: COX-2 activity is targeted by NSAIDs such as aspirin and natural inhibitors like calcitriol (Vitamin D).

Specifications:

Gene and Protein Information:

• Gene Symbol: PTGS2 (Prostaglandin-endoperoxide synthase 2).
• Protein Aliases: COX-2, Cyclooxygenase-2, PGH synthase 2.
• UniProt ID: P35354.

Advantages:

1. High Sensitivity: Detects low levels of COX-2 in various sample types.
2. Wide Dynamic Range: Covers both physiological and pathological COX-2 concentrations.
3. Reproducible Results: Low intra- and interassay variability.
4. Ready-to-Use Kit: Includes all necessary reagents and consumables for straightforward setup.
5. Validated Specificity: Exclusively recognizes human COX-2, including both natural and recombinant forms.

Use Cases:

• Monitoring COX-2 expression in inflammatory and cancer-related studies.
• Assessing the efficacy of anti-inflammatory drugs and COX-2 inhibitors.
• Investigating the role of COX-2 in neuroinflammation and neurodegenerative diseases.

This kit is a valuable tool for research in inflammation, oncology, pharmacology, and neuroscience. It provides reliable and precise quantitation of COX-2 levels, enabling deeper insights into its biological and pathological roles.