Invitrogen™ Malondialdehyde (MDA) Competitive ELISA Kit, 96 Tests

The Invitrogen™ Malondialdehyde (MDA) Competitive ELISA Kit is a sensitive and quantitative immunoassay designed for the measurement of malondialdehyde (MDA) in a wide range of biological samples. MDA is a well-established biomarker of lipid peroxidation and oxidative stress, making this kit an essential tool for research in oxidative damage, inflammation, toxicology, aging, cancer, cardiovascular disease, and metabolic disorders.

This multispecies competitive ELISA enables reliable detection of MDA in serum, plasma, cell lysates, and tissue homogenates, supporting translational and preclinical research across multiple biological models.

Intended Use

This kit is intended for the quantitative determination of malondialdehyde (MDA) concentrations in biological samples for research purposes only.

Not for diagnostic or therapeutic use.

Assay Principle

The assay is based on a competitive ELISA format. Microplate wells are pre-coated with MDA antigen. During the assay, free MDA in the sample competes with plate-bound MDA for binding to a biotinylated anti-MDA detection antibody.

After incubation, unbound components are removed by washing, and avidin-conjugated horseradish peroxidase (HRP) is added to bind the biotinylated antibody. Following a second incubation and wash step, TMB substrate is added, producing a colorimetric reaction. The reaction is stopped, and absorbance is measured using a microplate reader.

Because the assay is competitive, signal intensity is inversely proportional to the concentration of MDA present in the sample. Sample concentrations are calculated by comparison with a standard curve generated using provided MDA standards.

Key Features & Benefits

• Sensitive detection of lipid peroxidation marker MDA
• Wide assay range: 31.25 – 2000 ng/mL
• High analytical sensitivity: 18.75 ng/mL
• Excellent precision:
  • Intra-assay CV < 10%
  • Inter-assay CV < 10%
• Multispecies compatibility – independent of species origin
• Validated lot-to-lot consistency
• Comprehensive reagent set for streamlined workflows

Sample Types & Volumes

Performance Specifications

Kit Components

Each kit contains sufficient reagents for 96 determinations:

• Pre-coated 96-well microplate
• Malondialdehyde Standard
• Biotinylated Detection Antibody
• HRP Conjugate
• Standard & Sample Diluent
• Biotinylated Detection Antibody Diluent
• HRP Conjugate Diluent
• Wash Buffer
• TMB Substrate
• Stop Solution
• Plate Sealer

Storage & Shipping

• Shipping Conditions: Wet ice
• Storage: Refer to product documentation for component-specific storage temperatures
• Store reagents according to instructions to maintain assay performance

Target Information – Malondialdehyde (MDA)

Malondialdehyde (MDA) is a highly reactive α,β-unsaturated aldehyde formed as a byproduct of polyunsaturated fatty acid peroxidation. It is widely used as a biomarker for oxidative stress-induced lipid damage. MDA can form covalent adducts with proteins (advanced lipoxidation end products, ALEs) and DNA, contributing to cellular toxicity, mutagenesis, and disease progression. Elevated MDA levels are associated with oxidative stress in numerous pathological conditions and environmental exposures.

Applications

• Oxidative stress and lipid peroxidation research
• Inflammation and metabolic disorder studies
• Toxicology and environmental exposure assessment
• Aging and neurodegeneration research
• Cardiovascular and cancer research
• Antioxidant efficacy studies

The Invitrogen™ Malondialdehyde Competitive ELISA Kit (EEL160) provides a robust, sensitive, and reproducible solution for quantifying lipid peroxidation via MDA measurement in diverse biological samples. With excellent precision, multispecies compatibility, and rigorous validation, this kit is a valuable tool for researchers investigating oxidative damage and redox-related disease mechanisms across basic, translational, and applied research settings.