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Invitrogen™ Human Aspartate Aminotransferase (AST/GOT1) ELISA Kit, 96 Tests
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Human Aspartate Aminotransferase (AST/GOT1) ELISA Kit is a highly sensitive solid-phase sandwich ELISA assay designed for the quantitative detection of AST in human serum and plasma. AST (also known as GOT1) is a key enzyme involved in amino acid metabolism and is widely used as a biomarker for liver function and tissue damage.
This kit utilizes a pre-coated 96-well microplate and a matched antibody pair to ensure high specificity and reproducibility. The assay employs a biotin-labeled detection antibody and HRP-based colorimetric detection, enabling accurate measurement of AST concentration through absorbance reading.
Key Features
- High sensitivity with detection down to 0.19 ng/mL
- Quantitative detection across a broad assay range (0.31–20 ng/mL)
- Sandwich ELISA format for high specificity
- Pre-coated plate for simplified workflow
- Validated performance with low inter- and intra-assay variability (<10%)
- Compatible with standard microplate readers
- Complete kit with all necessary reagents included
Applications
- Liver function and hepatotoxicity studies
- Clinical and translational research
- Biomarker quantification in serum and plasma
- Metabolic and enzymatic pathway analysis
- Drug development and toxicology studies
Assay Principle
The assay is based on a sandwich ELISA format:
- Wells are pre-coated with a capture antibody specific to AST
- Samples are added and AST binds to the immobilized antibody
- A biotinylated detection antibody binds to a different epitope
- An HRP-conjugated antibody forms the detection complex
- Addition of TMB substrate generates a measurable color signal
- Signal intensity is directly proportional to AST concentration
Technical Specifications
| Parameter | Details |
|---|---|
| Detection Method | Colorimetric (HRP/TMB) |
| Assay Type | Sandwich ELISA |
| Analytical Sensitivity | 0.19 ng/mL |
| Assay Range | 0.31 – 20 ng/mL |
| Sample Types | Human serum, plasma |
| Sample Volume | 100 µL |
| Total Assay Time | ~3 hr 30 min |
| Hands-on Time | ~1 hr 20 min |
| Intra-assay CV | <10% |
| Inter-assay CV | <10% |
| Instrument | Absorbance microplate reader |
| Detector Antibody | Biotin |
| Label | HRP |
Kit Contents
- Pre-coated 96-well microplate
- AST standard
- Biotinylated detection antibody
- HRP conjugate
- Sample and reagent diluents
- Wash buffer
- TMB substrate
- Stop solution
- Plate sealer
Target Information
- Protein Name: GOT1 (Aspartate Aminotransferase)
- Protein Family: Pyridoxal phosphate-dependent aminotransferase
- Biological Role: Involved in amino acid metabolism, urea cycle, and TCA cycle
- Exists in both cytoplasmic (GOT1) and mitochondrial (GOT2) forms
Product Variant
| Catalog Number | Quantity |
|---|---|
| EEL029 | 96 tests |
Storage & Handling
- Store components as indicated in product documentation
- Ship under wet ice conditions
- Follow recommended storage conditions for individual reagents
The Invitrogen™ Human AST (GOT1) ELISA Kit provides a robust and sensitive solution for the quantitative analysis of aspartate aminotransferase in human samples. With its validated performance, high specificity, and complete reagent system, it enables researchers to generate reliable and reproducible results.
Ideal for biomarker research, liver function studies, and metabolic investigations, this kit is a valuable tool for laboratories focused on precision and consistency in protein quantification.