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Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit, 96 Tests
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Specifications:
| Application | Colorimetric Assay, Fluorescence-Based Assay | ||
| Storage Temperature | -20°C, 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
Sensitive fluorescence-based detection of ROS in live cells and fresh tissue samples
The Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit is a highly sensitive, quantitative assay for detecting and measuring ROS in fresh tissue samples and live cultured cells. ROS, such as superoxide anion, hydroxyl radical, and hydrogen peroxide, are critical mediators in both physiological signaling pathways and pathological processes including oxidative stress, inflammation, aging, and apoptosis.
The assay employs 2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA), a non-fluorescent, cell-permeable probe. Once inside the cell, DCFH-DA is hydrolyzed by intracellular esterases to form dichlorodihydrofluorescein (DCFH), which is then oxidized by ROS into highly fluorescent dichlorofluorescein (DCF). The resulting fluorescence signal (Ex/Em = 502/525 nm) is directly proportional to the ROS concentration in the sample.
Key Features
- High Sensitivity – Detects low levels of ROS in cellular and tissue systems
- Quantitative & Reproducible – Provides accurate ROS measurements with minimal variability
- Fast & Simple Workflow – 30–60 min assay time with minimal hands-on steps
- Versatile Readout – Compatible with microplate readers, spectrophotometers, and flow cytometers
- Live Cell & Tissue-Compatible – Non-destructive assay for real-time ROS monitoring
Applications
- Oxidative Stress Research – Monitor ROS generation under physiological or pathological conditions
- Cell Signaling Studies – Quantify ROS involvement in signaling pathways
- Toxicology & Drug Screening – Assess compound-induced oxidative stress in cells
- Neurodegenerative & Cardiovascular Research – Study ROS-associated disease mechanisms
- Antioxidant Efficacy Testing – Evaluate ROS scavenging capacity of therapeutic agents
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | EEA019 |
| Detection Method | Fluorescence (Ex/Em 502/525 nm) |
| Assay Format | 96 tests |
| Sample Types | Live cultured cells, fresh tissue samples |
| Incubation Time | 30–60 minutes |
| Total Assay Time | ~60 minutes |
| Equipment | Microplate reader, spectrophotometer, or flow cytometer |
| Shipping Conditions | Dry ice, 2–8°C |
| Storage | Reagent 1: –20°C, Reagent 2: 2–8°C |
| Regulatory Status | Research Use Only (RUO) |
The Invitrogen™ Reactive Oxygen Species (ROS) Fluorometric Assay Kit is an essential tool for quantifying ROS activity with high sensitivity and reproducibility. Its fast, easy-to-use format makes it ideal for oxidative stress research, antioxidant screening, toxicology, and disease pathway studies.