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Thermo Scientific™ Oxoid™ Brain Heart Infusion Broth (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Microbiology grade | ||
The Thermo Scientific™ Brain Heart Infusion Broth (Dehydrated) is a highly nutritious, well-buffered culture medium designed for the cultivation of fastidious organisms, including streptococci, Neisseria, and other challenging microbes. This versatile medium is ideal for use in diagnostic, clinical, and research laboratories.
Key Features
- Highly Nutritious: Supports the growth of a wide range of fastidious organisms, including bacteria and fungi.
- Buffered Medium: Ensures consistent pH stability for optimal microbial growth.
- Versatile Applications:
- Blood culture for the isolation of pathogenic fungi.
- Growth and suspension medium for staphylococci in coagulase production tests.
- General-purpose medium for microbiological research and diagnostics.
- Customizable: Can be enriched with 10% defibrillated blood to promote fungal growth.
Specifications
| Attribute | Details |
|---|---|
| Description | Brain Heart Infusion Broth |
| Form | Powder |
| Product Type | Dehydrated Culture Media |
| Quantity | 500 g (Each) |
| Yield | 13.5 L medium |
| Weight (Metric) | 500 g |
| Unit Size | Each |
| Applications | Diagnostic and clinical microbiology |
| Compatibility | For In Vitro Diagnostic Use |
The composition of Thermo Scientific™ Brain Heart Infusion (BHI) Broth (Dehydrated) is formulated to provide the necessary nutrients and buffering capacity to support the growth of fastidious microorganisms, such as streptococci, Neisseria, and pathogenic fungi. The typical formulation is as follows (per liter of prepared medium):
Typical Composition (g/L)
| Component | Amount (g/L) | Function |
|---|---|---|
| Brain Infusion (solids) | 200.0 mg | Provides essential nutrients (amino acids, peptides). |
| Heart Infusion (solids) | 250.0 mg | Supplies additional growth factors for fastidious organisms. |
| Peptone | 10.0 g | Source of nitrogen, peptides, and amino acids. |
| Dextrose (Glucose) | 2.0 g | Energy source for microbial metabolism. |
| Sodium Chloride (NaCl) | 5.0 g | Maintains osmotic balance. |
| Disodium Phosphate (Na₂HPO₄) | 2.5 g | Acts as a buffering agent to maintain pH stability. |
Final pH
- 7.4 ± 0.2 at 25°C after preparation.
Optional Additives:
- Defibrillated Blood (10%): Enhances the medium for the isolation and cultivation of pathogenic fungi.
Applications
- Bacterial Cultivation:
- Ideal for streptococci and Neisseria.
- Supports fastidious bacterial growth for clinical and diagnostic work.
- Pathogenic Fungi:
- Enriched with 10% defibrillated blood, it is suitable for isolating and cultivating pathogenic fungi.
- Coagulase Production Testing:
- Used as a growth and suspension medium for staphylococci in coagulase tests.
- General Microbiological Studies:
- Suitable for research and routine diagnostic applications requiring a robust growth medium.
Preparation Instructions
- Dissolve the required amount of dehydrated medium in distilled water.
- Sterilize by autoclaving at 121°C for 15 minutes.
- Allow the medium to cool before use.
- (Optional) Add 10% defibrillated blood for fungal cultivation.
Advantages
- Wide Applicability: Effective for bacterial and fungal cultivation in diverse clinical and laboratory settings.
- High Yield: A single 500 g container prepares 13.5 L of medium, offering cost-effective, large-scale utility.
- Reliable Brand: Produced under the trusted Thermo Scientific™ Oxoid™ and Remel™ lines.
Notes
- Intended for in vitro diagnostic use only.
Ordering Information
| Catalog Number | Weight | Yield | Unit Size |
|---|---|---|---|
| CM1135B | 500 g | 13.5 L | Each |