Thermo Scientific™ Oxoid™ EC Broth with MUG (Dehydrated)

Thermo Scientific™ Oxoid™ EC Broth with MUG is a selective enrichment medium designed for the detection and differentiation of Escherichia coli and fecal coliforms in food, environmental, and dairy samples. The addition of MUG (4-methylumbelliferyl-β-D-glucuronide) enables the detection of β-glucuronidase (GUD) activity, which is specific to E. coli, providing a fluorescent end-product for rapid and reliable identification.

Key Features

1. Selectivity

• Contains Bile Salts No. 3 to inhibit the growth of Gram-positive bacteria, such as bacilli and fecal streptococci, ensuring selectivity for Escherichia coli.

2. MUG Fluorescence Detection

• MUG is cleaved by β-glucuronidase, an enzyme produced by E. coli, resulting in a fluorescent end-product under UV light, making it easy to confirm the presence of E. coli.

3. Versatile Applications

• Recommended for:
  • Enumeration of presumptive E. coli in milk and dairy products.
  • Confirmation of E. coli in food and environmental samples.
  • Detection of E. coli from shellfish and meat, as per FDA/BAM guidelines.

4. Reliable Results

• Produces dependable results within 24–48 hours, allowing rapid detection and confirmation of fecal contamination.

5. Regulatory Compliance

• Compliant with FDA/BAM standards, ensuring suitability for regulatory testing.

Applications

1. Food Safety Testing:
   • Detect and differentiate Escherichia coli in raw and processed foods.
2. Water Quality Monitoring:
   • Identify E. coli contamination in drinking water and recreational water sources.
3. Dairy Industry:
   • Confirm the presence of presumptive E. coli in milk and milk products.
4. Environmental Testing:
   • Monitor fecal contamination in wastewater and sewage.
5. Shellfish and Meat Testing:
   • Compliant with FDA/BAM methods for E. coli confirmation in shellfish and meat samples.

Composition (Typical Formula)

Preparation Instructions

1. Suspend the required amount of EC Broth with MUG powder in distilled water (e.g., 37.0g for 1L medium).
2. Heat gently to dissolve completely.
3. Dispense into suitable tubes or containers.
4. Sterilize by autoclaving at 121°C for 15 minutes.

Advantages

1. Enhanced Detection with MUG:
   • The fluorescent reaction provides a rapid and specific method for identifying E. coli.
2. Wide Range of Applications:
   • Suitable for food, water, dairy, and environmental testing.
3. Rapid and Reliable:
   • Produces results in 24–48 hours, enabling timely interventions.
4. Ease of Use:
   • Gas production and fluorescence offer straightforward indicators for E. coli detection.

Quality Control

Specifications

• Form: Powder
• Yield: 500g makes approximately 13.5L of medium
• Certifications/Compliance: FDA
• Unit Size: Each

Storage and Shelf Life

1. Store the dehydrated medium at 10–30°C in a tightly sealed container.
2. Use before the expiration date printed on the label.

Why Choose Thermo Scientific™ Oxoid™ EC Broth with MUG?

Thermo Scientific™ Oxoid™ EC Broth with MUG offers a reliable and selective solution for detecting Escherichia coli in a wide range of applications. Its integration of MUG fluorescence technology provides rapid, accurate identification, making it ideal for microbiological laboratories focused on food safety, water quality, and public health.