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Thermo Scientific™ Oxoid™ EC Broth (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™ | Target Organism Class | Escherichia Coli |
| Product Grade | Microbiology grade | ||
Thermo Scientific™ Oxoid™ EC Broth is a selective enrichment medium specifically formulated for the detection and differentiation of Escherichia coli and fecal coliforms from food, environmental, and dairy samples. The medium is particularly useful for confirmatory testing of E. coli using gas production as a presumptive indicator and is compliant with industry standards such as ISO and NMKL.
Key Features
1. Selectivity
- The inclusion of Bile Salts No. 3 inhibits Gram-positive bacteria, including bacilli and fecal streptococci, making the medium selective for E. coli and fecal coliforms.
2. Versatility
- Suitable for the detection of E. coli from:
- Food samples
- Environmental water samples
- Milk and dairy products using the Most Probable Number (MPN) technique.
3. Easy-to-Read Results
- Gas production from lactose fermentation provides presumptive evidence of coliform presence, simplifying interpretation.
4. Reliable and Rapid
- Produces dependable results within 24–48 hours, ensuring timely detection of fecal contamination.
5. Industrial Compliance
- Conforms to:
- ISO Standards
- NMKL Standards
- APHA Standard Methods for water and wastewater examination.
Applications
- Food Safety Testing:
- Detect and differentiate fecal coliforms in processed and raw foods.
- Water Quality Monitoring:
- Confirm Escherichia coli contamination in environmental and drinking water samples.
- Dairy Industry:
- Enumeration of presumptive E. coli in milk and dairy products.
- Public Health:
- Monitor fecal contamination in wastewater and sewage.
- Veterinary Microbiology:
- Assess contamination in animal feed and water sources.
Composition (Typical Formula)
| Component | gm/Litre |
|---|---|
| Tryptone | 20.0 |
| Lactose | 5.0 |
| Bile Salts No. 3 | 1.5 |
| Di-potassium Phosphate | 4.0 |
| Mono-potassium Phosphate | 1.5 |
| Sodium Chloride | 5.0 |
| pH | 6.9 ± 0.2 @ 25°C |
Preparation Instructions
- Suspend the required amount of EC Broth powder in distilled water (e.g., 37.0g for 1L medium).
- Heat gently to dissolve completely.
- Dispense into tubes or containers.
- Sterilize by autoclaving at 121°C for 15 minutes.
Advantages
- Enhanced Selectivity:
- Suppresses non-target organisms, ensuring accurate detection of fecal coliforms and E. coli.
- Wide Applications:
- Suitable for various industries, including food safety, water quality, and dairy testing.
- Rapid Results:
- Delivers reliable results within 24-48 hours, enabling timely interventions.
- Ease of Use:
- Gas production offers a straightforward presumptive test for coliform detection.
Quality Control
| Control | Expected Result |
|---|---|
| Positive Control | |
| Escherichia coli ATCC® 25922 | Turbid growth with gas production |
| Negative Control | |
| Staphylococcus aureus ATCC® 25923 | No growth |
Specifications
- Form: Powder
- Yield: 500g makes approximately 13.5L of medium
- Unit Size: Each
- Certifications/Compliance: ISO, NMKL standards
Storage and Shelf Life
- Store the dehydrated medium at 10–30°C.
- Keep in a tightly sealed container to avoid moisture absorption.
- Use before the expiration date printed on the label.
Why Choose Thermo Scientific™ Oxoid™ EC Broth?
Thermo Scientific™ Oxoid™ EC Broth is a reliable, industry-standard medium for detecting Escherichia coli and fecal coliforms. Its selectivity, rapid detection, and compliance with international standards make it an essential tool for microbiological laboratories focused on food safety, water quality, and public health.