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Thermo Scientific™ Oxoid™ Dichloran-Glycerol (DG18) Agar Base (Dehydrated), 500g
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Specifications:
| Application | Industrial Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, Oxoid | ||
| Product Grade | Microbiology grade | ||
Thermo Scientific™ Oxoid™ Dichloran-Glycerol (DG18) Agar Base is a selective, low water activity microbiological culture medium designed for the isolation and enumeration of yeasts and molds associated with food spoilage, particularly in dried and semi-dried food products. This dehydrated medium is formulated according to the Hocking and Pitt method and is widely used in food microbiology laboratories for accurate fungal detection and colony counting.
The incorporation of dichloran restricts the spreading of fast-growing fungi, while glycerol lowers water activity to enhance recovery of xerophilic and xerotolerant molds, ensuring improved detection of fungal contaminants in low-moisture foods.
Key Features
- Selective culture medium for isolation and enumeration of yeasts and molds
- Low water activity formulation enhances recovery of xerophilic fungi
- Contains dichloran to inhibit spreading and improve colony differentiation
- Facilitates accurate enumeration by preventing colony overgrowth
- Recommended for testing dried and semi-dried food products
- Dehydrated powder format for convenient preparation
- Complies with industrial microbiology standards
Principle of Operation
DG18 Agar Base contains:
- Dichloran, which inhibits rapid spreading of mucoraceous fungi and restricts colony size
- Glycerol (16%), which lowers water activity from 0.999 to approximately 0.95, promoting selective growth of xerophilic molds
- Nutrient components that support fungal growth while allowing clear colony visualization
This selective environment allows better recovery and accurate enumeration of molds and yeasts that thrive in low-moisture food environments.
Applications
DG18 Agar Base is ideal for:
- Enumeration of yeasts and molds in food products
- Detection of fungal contamination in dried foods
- Quality control in food manufacturing laboratories
- Environmental and industrial microbiology testing
- Microbial analysis of low-moisture food products
Commonly tested products include:
- Dried fruits
- Cereals and grains
- Nuts and spices
- Confectionery
- Dried meat and fish products
Technical Specifications
| Parameter | Specification |
|---|---|
| Product Name | Dichloran-Glycerol (DG18) Agar Base |
| Catalog Number | CM0729B |
| Brand | Thermo Scientific™ Oxoid™ |
| Form | Dehydrated Powder |
| Quantity | 500 g |
| Yield | Up to 15.9 L of prepared medium |
| Water Activity (aW) | Approximately 0.95 |
| Selective Agent | Dichloran |
| Product Type | Microbiological Culture Agar |
| Compliance | NMKL Industrial Standards |
| Application | Yeast and mold isolation and enumeration |
| Storage | Store in a cool, dry place |
Advantages Over Conventional Media
Compared to higher water activity media such as DRBC Agar, DG18 Agar Base provides:
- Improved recovery of xerophilic fungi
- Better colony separation and counting
- Enhanced detection of molds such as Aspergillus penicillioides and Wallemia sebi
- Reduced interference from rapidly spreading fungal species
Preparation Guidelines
The dehydrated powder is reconstituted in distilled water according to manufacturer instructions and sterilized prior to use.
Thermo Scientific™ Oxoid™ Dichloran-Glycerol (DG18) Agar Base is a reliable and widely used selective culture medium for detecting and enumerating yeasts and molds in dried and low-moisture food products. Its low water activity formulation and dichloran-based selectivity ensure accurate fungal recovery, making it an essential medium for food microbiology, quality control, and contamination monitoring laboratories.