Thermo Scientific™ Oxoid™ Tryptose Blood Agar Base (Dehydrated)

The Thermo Scientific™ Oxoid™ Tryptose Blood Agar Base (Dehydrated) is a highly nutritious medium designed for the cultivation and isolation of fastidious microorganisms. When supplemented with blood, it supports the growth of demanding organisms and allows for the determination of hemolytic reactions. Its formulation, developed by Casman, has been refined to eliminate dextrose, which can interfere with hemolytic reactions, ensuring accurate and reliable results.

Key Features

1. Highly Nutritious:
   • Contains tryptose and other essential nutrients to support the growth of fastidious microorganisms.
2. Supports Hemolytic Reactions:
   • Supplementation with blood enables the detection of alpha, beta, and gamma hemolysis.
3. Versatile Application:
   • Can be used without blood for good to excellent growth of many microorganisms.
   • Addition of yeast extract (1g per liter) enhances the growth of organisms like Neisseria meningitidis and Streptococcus pneumoniae.
4. Refined Formulation:
   • Dextrose-free to prevent interference with hemolytic reactions.
5. Wide Compatibility:
   • Ideal for clinical, food, and environmental microbiology.

Specifications

Applications

1. Clinical Microbiology:
   • Isolation and identification of fastidious pathogens such as Streptococcus pneumoniae and Neisseria meningitidis.
2. Food Microbiology:
   • Detection of hemolytic organisms in food and beverage samples.
3. Research:
   • Supports studies on hemolytic reactions and the growth characteristics of demanding bacteria.

Preparation Instructions

1. Suspend 30g of Tryptose Blood Agar Base powder in 1 liter of distilled water.
2. Heat gently to dissolve completely.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool to approximately 50°C and aseptically add 5-10% sterile defibrinated blood if required.
5. Mix thoroughly and pour into sterile Petri dishes.

Advantages

• Reliable Hemolysis Detection: Optimized for accurate identification of hemolytic reactions.
• Enhanced Growth Support: Highly nutritious formulation supports fastidious organisms.
• Flexible Usage: Effective with or without blood supplementation.
• Consistent Results: Refined formula eliminates dextrose interference, ensuring reliable outcomes.

Packaging

• Quantity: 500g of dehydrated powder.
• Yield: Prepares 16.7L of medium.

Why Choose Tryptose Blood Agar Base?

• Trusted for its performance in supporting the growth of fastidious organisms.
• Ideal for clinical and food microbiology applications.
• Backed by Thermo Scientific’s commitment to quality and innovation.