Thermo Scientific™ Oxoid™ Urea Broth Base (Dehydrated)

The Thermo Scientific™ Oxoid™ Urea Broth Base (Dehydrated) is a liquid modification of Christensen medium, specifically designed to differentiate urease-producing Enterobacteriaceae from Salmonella and Shigella species during routine microbiological testing of rectal swabs and fecal samples. This medium offers an efficient and rapid method for identifying urease-positive organisms such as Proteus spp., saving time and resources in diagnostic workflows.

Key Features

1. Efficient Differentiation:
   • Quickly differentiates urease-positive Proteus spp. from Salmonella and Shigella groups, reducing unnecessary follow-up testing.
2. Liquid Medium for Easy Handling:
   • The fluid formulation allows inoculation with a Pasteur pipette, simplifying workflows and reducing contamination risks.
3. Rapid Results:
   • Positive urease reactions are visible within 2-5 hours at 35°C, enhancing laboratory efficiency.
4. Reliable and Clear Detection:
   • Provides distinct color changes for easy interpretation of urease activity.

Specifications

Applications

1. Clinical Microbiology:
   • Differentiates urease-producing Enterobacteriaceae, such as Proteus, from non-urease producers like Salmonella and Shigella in fecal and rectal samples.
2. Food Microbiology:
   • Detects urease activity in isolates from foodborne samples for quality control.
3. Environmental Testing:
   • Identifies urease-positive organisms in water and soil samples.
4. Research:
   • Studies bacterial urease production and metabolic pathways.

Preparation Instructions

1. Suspend 9g of Urea Broth Base powder in 1 liter of distilled water.
2. Heat gently to dissolve completely.
3. Sterilize by autoclaving at 121°C for 15 minutes.
4. Cool to 50°C, and aseptically add 40% Urea Solution (SR0020K) to a final concentration as directed.
5. Mix thoroughly and dispense into sterile containers.

Advantages

• Time-Saving: Detects urease-positive organisms rapidly, within 2-5 hours.
• Cost-Effective: Reduces unnecessary testing by eliminating non-target organisms.
• Easy to Use: Liquid medium is simple to inoculate and handle with minimal contamination risk.
• Reliable Results: Clear-cut color changes ensure accurate interpretation of urease activity.

Packaging

• Quantity: 500g of dehydrated powder.
• Yield: Prepares 55.6L of medium.

Why Choose Urea Broth Base?

• Optimized for rapid differentiation of urease-positive Enterobacteriaceae.
• Enhances laboratory efficiency with easy handling and clear results.
• Trusted for its reliability in clinical, food, and environmental microbiology.