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Thermo Scientific™ Oxoid™ Macconkey Broth (Dehydrated)
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Specifications:
| Application | Microbiology | ||
| Storage Temperature | Ambient | ||
| Product Type | Culture Medium | Forms | Powder |
| Product Brand | Oxoid | Target Organism Class | Escherichia Coli, coliform bacteria |
| Product Grade | Microbiology grade | Formula | For 12.5L medium |
The Thermo Scientific™ Oxoid™ MacConkey Broth is a selective and differential medium designed for the detection and isolation of coliform bacteria in water, milk, and other specimens. It is widely regarded as the standard medium for primary coliform isolation, recommended by authoritative organizations like the Public Health Laboratory Service Water Committee and the World Health Organization (WHO).
MacConkey Broth is formulated to differentiate lactose-fermenting coliforms based on acid production, indicated by a color change due to the presence of neutral red as a pH indicator. Its selective nature suppresses non-coliform organisms through the inclusion of bile salts.
Key Features
1. Selective Properties
- Bile salts: Inhibits Gram-positive bacteria, allowing selective growth of coliforms and other Gram-negative organisms.
2. Differential Properties
- Lactose fermentation:
- Lactose-fermenting coliforms produce acid, causing the medium to turn pink/red due to the neutral red pH indicator.
- Non-lactose fermenters leave the broth unchanged.
3. Versatile Applications
- Approved for water testing by public health authorities.
- Recommended for milk grading and dairy industry microbiology.
4. Conforms to International Standards
- Formulated according to guidelines from:
- Public Health Laboratory Service (PHLS) Water Committee.
- World Health Organization (WHO).
- UK Department of Health for milk grading.
Composition (Typical Formula per Liter)
| Component | Amount (g/L) | Purpose |
|---|---|---|
| Peptone | 20.0 | Provides nitrogen and carbon for bacterial growth. |
| Lactose | 10.0 | Differentiates lactose fermenters from non-fermenters. |
| Bile Salts | 5.0 | Selectively inhibits Gram-positive organisms. |
| Sodium Chloride | 5.0 | Maintains osmotic balance in the medium. |
| Neutral Red | 0.075 | pH indicator to detect acid production. |
| pH | 7.4 ± 0.2 | Optimal pH for coliform growth and differentiation. |
Applications
1. Water Testing
- Detects and enumerates coliform bacteria as indicators of fecal contamination and water quality.
2. Milk Grading
- Ensures compliance with public health standards by identifying coliform contamination in milk and dairy products.
3. Food Microbiology
- Assesses hygiene and contamination levels in food processing and storage facilities.
4. Environmental Monitoring
- Evaluates sewage and wastewater for potential health risks by detecting coliform presence.
Advantages
1. High Selectivity
- Effectively suppresses non-coliform organisms for more accurate coliform detection.
2. Easy-to-Read Results
- Clear color change allows rapid identification of lactose fermenters.
3. Internationally Recognized
- Trusted and recommended by WHO, PHLS, and the UK Department of Health for water and milk testing.
4. Reliable and Reproducible
- Consistent formulation ensures repeatable results across a variety of applications.
How It Works
- Preparation:
- Dissolve the dehydrated medium as per manufacturer instructions.
- Autoclave at 121°C for 15 minutes.
- Inoculation:
- Add the sample (e.g., water or milk) to the prepared broth.
- Incubation:
- Incubate at 35–37°C for 18–48 hours under aerobic conditions.
- Interpretation:
- Positive for coliforms: Broth turns pink/red due to acid production from lactose fermentation.
- Negative for coliforms: No color change.
Why Choose MacConkey Broth?
- Compliant with Standards: Formulated to meet public health guidelines.
- Broad Applicability: Ideal for water, milk, and environmental testing.
- Reliable Results: High-quality medium ensures accurate coliform detection.
The Thermo Scientific™ Oxoid™ MacConkey Broth (Dehydrated) is an essential tool for laboratories involved in water quality analysis, milk grading, and environmental monitoring, providing reliable and accurate results for coliform detection.