The 5-(and-6)-Carboxyfluorescein Diacetate, Succinimidyl Ester (CFDA, SE; CFSE) is a fluorescein-based, fixable, and cell-permeant tracer for long-term cell labeling. It is widely used in flow cytometry and fluorescence microscopy to monitor cell division, viability, and proliferation due to its excellent stability and fluorescence retention.
Key Features
- Long-Term Labeling:
- Ideal for tracking cell proliferation and viability over extended periods.
- Fixable Tracer:
- Covalently binds to intracellular proteins, making it resistant to wash steps and fixation.
- Versatile Compatibility:
- Compatible with flow cytometry and fluorescence microscopy for multi-application workflows.
- Easy-to-Dissolve:
- Soluble in DMSO, ensuring easy preparation for staining protocols.
- Convenient Packaging:
- Available as a 25 mg solid in a single vial for multiple experimental setups.
- Broad Application Range:
- Used for cell tracking, division monitoring, and intracellular labeling in diverse cell types.
Specifications
| Parameter | Details |
|---|
| Excitation/Emission Wavelength | 492/517 nm |
| Dye Type | CFDA SE (CFSE) |
| Form | Solid |
| Quantity | 25 mg |
| Solubility | DMSO |
| Detection Method | Fluorescence |
| For Use With (Equipment) | Fluorescence Microscope, Flow Cytometer |
| Storage Conditions | Store at -5°C to -30°C |
| Shipping Condition | Room Temperature |
| Label Type | Classic Dyes |
| Applications | Cell division tracking, viability assays, long-term cell labeling |
Applications
- Cell Proliferation Tracking:
- Used in flow cytometry for monitoring cell division cycles by tracking fluorescence intensity dilution.
- Viability Assays:
- Labels viable cells with strong fluorescence retention.
- Intracellular Labeling:
- Covalent attachment to proteins ensures robust labeling for microscopy studies.
- Immune Studies:
- Tracks immune cell proliferation and migration in immunological experiments.
- Longitudinal Studies:
- Suitable for long-term tracking of labeled cells over days or weeks.
Advantages
| Feature | Benefit |
|---|
| Fixable Label | Resistant to fixation and washing steps. |
| Long-Term Stability | Tracks cell division over multiple generations. |
| Bright Fluorescence | High signal-to-noise ratio for clear detection. |
| Versatile Application | Compatible with microscopy and flow cytometry. |
| Easy-to-Use Formulation | Dissolves quickly in DMSO for streamlined protocols. |
Comparison with Competitor Products
| Feature | Invitrogen™ 5(6)-CFDA, SE (CFSE) | Sigma-Aldrich CFSE | Abcam CFSE | Thermo Fisher CellTrace CFSE |
|---|
| Fluorescence Intensity | High, robust signal | Moderate | High | Very high, optimized for flow cytometry |
| Fixable Properties | Yes | Yes | Yes | Yes |
| Cell Labeling Retention | Long-term tracking | Medium-term tracking | Long-term tracking | Long-term tracking |
| Solubility | DMSO | DMSO | DMSO | DMSO |
| Packaging Quantity | 25 mg | 10–50 mg | 10–50 mg | 1 kit |
| Application Versatility | High | Moderate | High | Very high |
| Cost Per Unit | Moderate | Low | Moderate | Higher due to kit format |
| Technical Support | Comprehensive | Standard | Strong | Extensive |
Competitor Advantages vs. Invitrogen™ CFSE
- Sigma-Aldrich CFSE:
- Lower price but slightly less fluorescence intensity.
- Suitable for budget-conscious labs with simpler needs.
- Abcam CFSE:
- High fluorescence but lacks Invitrogen™’s broader product integration and advanced support.
- Thermo Fisher CellTrace CFSE:
- Extremely high performance but comes at a higher cost due to additional kit components.
Suggested Pairing
- Vybrant CFDA-SE Cell Tracer Kit (V12883):
- Streamlines protocols for convenient use with additional reagents included.
- Flow Cytometry Instruments:
- Compatible with Invitrogen™ Attune™ NxT Flow Cytometer for advanced analyses.
- Fluorescence Microscopes:
- Optimized for Invitrogen™ EVOS™ imaging systems.
https://www.labmartgh.com/shop/product/c1157-invitrogen-tm-5-6-cfda-se-cfse-25mg-28476
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