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Specifications:
| Application | Cold chain Storage |
| Storage Temperature | Room Temperature |
| Product Type | Storage container |
| Product Brand | Biologix |
| Product Grade | Molecular Biology |
The Biologix® Freezing Container is an efficient tool designed for controlled-rate freezing of cryogenic vials. It employs a programmed cooling process to minimize damage to cellular samples, ensuring sample integrity during the freezing process. With built-in foam insulation and compatibility with a variety of vial sizes, this container is ideal for laboratory applications requiring precise sample freezing.
Key Features:
- Controlled Freezing Rate:
- Maintains a consistent cooling rate of -1°C (-33.8°F) per minute, ideal for gradual freezing of cell samples.
- Programmed Cooling Process:
- Reduces damage to cellular structures and internal liquids caused by rapid freezing.
- Construction:
- Container Material: Polycarbonate for durability and resistance to low temperatures.
- Cap and Tube Holder Material: High-density polyethylene (HDPE) for chemical resistance and structural stability.
- Sample Compatibility:
- 90-7218: 18 holes (14.55 mm pore size), compatible with cryogenic vials of 1.0 mL, 1.2 mL, 1.5 mL, 1.8 mL, and 2.0 mL.
- 90-7312: 12 holes (14.55 mm pore size), compatible with cryogenic vials of 1.0 mL, 1.2 mL, 1.5 mL, 1.8 mL, 2.0 mL, and 5.0 mL.
- Isopropanol Integration:
- Pre-fill 250 mL of isopropanol before adding cryogenic vials to ensure controlled freezing.
- Storage and Maintenance:
- Store at room temperature when not in use.
- Replace isopropanol after every fifth use for consistent performance.
- Freezing Environment:
- Place the container in a -80°C (-176°F) mechanical freezer for optimal freezing.
Specifications:
- Freezing Rate: -1°C (-33.8°F) per minute.
- Material: Polycarbonate container; HDPE cap and tube holder.
- Capacity:
- 90-7218: 18 vials.
- 90-7312: 12 vials.
- Pore Size: 14.55 mm.
- Vial Compatibility: 1.0 mL to 5.0 mL cryogenic vials.
Usage Instructions:
- Preparation:
- Inject 250 mL of isopropanol into the container before use.
- Load cryogenic vials into the designated holes.
- Freezing Process:
- Place the container in a -80°C mechanical freezer.
- Allow the programmed freezing rate to proceed (-1°C/min).
- Post-Use Care:
- Store at room temperature when not in use.
- Replace isopropanol after every fifth use to maintain freezing efficiency.
Advantages:
- Precise and controlled freezing minimizes cell damage.
- Durable and chemically resistant construction.
- Compatible with a wide range of cryogenic vial sizes.
- Easy to use and maintain with replaceable isopropanol.
Applications:
- Freezing of cell cultures.
- Cryopreservation of biological samples.
- Controlled freezing for tissue samples.
- Laboratory use for maintaining sample integrity during freezing.
Comparison of the Biologix® Freezing Container, Mr. Frosty™ Freezing Container, and Corning® CoolCell® Freezing Containers:
| Feature | Biologix® Freezing Container | Mr. Frosty™ Freezing Container | Corning® CoolCell® Freezing Containers |
|---|---|---|---|
| Cooling Rate | -1°C/minute | -1°C/minute | -1°C/minute |
| Capacity | - 90-7218: 18 vials (1.0–2.0 mL) - 90-7312: 12 vials (1.0–5.0 mL) | - 5100-0001: 18 vials (1.0–2.0 mL) - 5100-0036: 12 vials (3.6 mL) - 5100-0050: 12 vials (4.5–5.0 mL) | - CoolCell LX: 12 vials (1.0–2.0 mL) - CoolCell 5 mL LX: 12 vials (3.5–5.0 mL) - CoolCell FTS30: 30 vials (1.0–2.0 mL) |
| Materials | - Container: Polycarbonate - Cap & Tube Holder: High-Density Polyethylene (HDPE) | - Container: Polycarbonate - Closure & Tube Holder: High-Density Polyethylene (HDPE) - Foam Insert | - Uniform-Density Cross-Linked Polyethylene Foam - Solid-State Core |
| Alcohol Requirement | Requires 250 mL of isopropanol; replace after every fifth use | Requires isopropyl alcohol; fill to the indicated line; replace after five uses | Alcohol-free operation |
| Freezer Compatibility | Place in a -80°C mechanical freezer | Place in a -70°C to -80°C mechanical freezer | Place in a -75°C to -80°C mechanical freezer or dry ice locker |
| Design Features | Built-in foam layer for insulation | Screw-top lid secures samples; foam insert prevents vial contact with alcohol | Radial vial symmetry; consistent and reproducible freezing profiles |
| Maintenance | Store at room temperature when not in use; replace isopropanol after fifth use | Store at room temperature when not in use; replace isopropyl alcohol after five uses | Minimal maintenance; no alcohol replacement needed |
| Advantages | - Controlled freezing rate - Compatible with various vial sizes | - Controlled freezing rate - Secure sample containment | - Alcohol-free - Consistent freezing profiles - Various capacities and colors available |
| Considerations | - Requires isopropanol handling and replacement | - Requires isopropyl alcohol handling and replacement | - Higher initial cost compared to alcohol-based containers |
- For: 18 cryogenic tubes (1.0 - 2.0 mL) 12 cryogenic tubes (4.5 - 5.0 mL)
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