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Invitrogen™ X-Gal (5-Bromo-4-Chloro-3-Indolyl β-D-Galactopyranoside), 1g
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Specifications:
| Application | Colorimetric Assay | ||
| Storage Temperature | -5 to -30°C | ||
| Product Type | Enzymes and Substrates | Forms | Solid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | Formula | C₁₄H₁₅BrClNO₆ |
Gold-standard chromogenic substrate for β-galactosidase detection
Invitrogen™ X-Gal is a widely used chromogenic substrate for β-galactosidase detection in molecular biology, histochemistry, and cell biology applications. Upon enzymatic hydrolysis by β-galactosidase, X-Gal is cleaved to produce galactose and 4-chloro-3-bromo-indigo, an insoluble dark blue precipitate that marks the site of enzymatic activity.
This substrate is cell-permeant and compatible with fixed cell and tissue samples, allowing detection even after fixation with glutaraldehyde. X-Gal is the gold-standard reagent for lacZ reporter assays, blue/white colony screening, and β-galactosidase-based immunohistochemical techniques.
Key Features
- High-contrast chromogenic detection – Produces an intense blue precipitate at enzymatic activity sites
- Cell-permeant – Suitable for live or fixed cell applications
- Fixation-compatible – Retains β-galactosidase activity after glutaraldehyde fixation
- Versatile – For histochemistry, molecular biology, and immunohistochemical assays
- Highly Specific – β-galactosidase selectively hydrolyzes β-D-galactopyranosides with minimal cross-reactivity
Applications
- lacZ reporter gene assays in eukaryotic and prokaryotic systems
- Blue/white colony screening in molecular cloning workflows
- Histochemical β-galactosidase staining in cells and tissues
- Immunohistochemistry with β-galactosidase-conjugated detection reagents
- Characterization of glycosidase activity in microorganisms and mammalian organelles
Specifications
| Parameter | Details |
|---|---|
| Catalog Number | B1690 |
| Chemical Name | 5-Bromo-4-Chloro-3-Indolyl β-D-Galactopyranoside |
| Quantity | 1 g |
| Form | Solid |
| Detection Method | Colorimetric |
| Cell Permeability | Yes |
| Substrate Type | β-galactosidase substrate |
| Storage Conditions | –5 °C to –30 °C |
| Shipping Conditions | Room temperature |
| Regulatory Status | Research Use Only (RUO) |
Invitrogen™ X-Gal remains the trusted benchmark chromogenic substrate for β-galactosidase detection, delivering high sensitivity, compatibility with fixed samples, and strong visual contrast. It is a key reagent in lacZ reporter assays, blue/white screening, and β-galactosidase histochemistry.