Invitrogen™ Luminex™ 200™ Instrument System

High-Throughput, Multiplexed Analysis for Gene and Protein Expression Using Flow Cytometry-Based Bead Technology

The Invitrogen™ Luminex™ 200™ Instrument System is a flow cytometry-based multiplexing platform that allows researchers to analyze up to 100 analytes simultaneously in a single well. Powered by Luminex xMAP® technology, this robust, versatile instrument integrates bead-based assays with digital signal processing to deliver high-throughput, reproducible quantification of protein and gene expression targets across a variety of applications.

With a read speed of up to 12,800 tests per hour, this system supports ProcartaPlex™ multiplex immunoassays and QuantiGene Plex™ gene expression assays, making it an ideal choice for biomarker discovery, immunology, and genomics research.

Key Features & Benefits

• True Multiplexing Power
  Perform up to 100 different tests in a single reaction volume using uniquely color-coded magnetic microspheres.
• High Throughput Performance
  Read an entire 96-well plate in ≤45 minutes, processing up to 12,800 tests per hour.
• Flexible Data Handling
  Compatible with optional software modules, including 21 CFR Part 11 compliance, automation support, and LIMS/LIS integration.
• Rapid Setup & Shutdown
  Daily start-up and shutdown procedures can be completed in under 30 minutes.
• Advanced Optical System
  Uses dual-laser optics to identify bead sets and quantify analytes via fluorophore-tagged detection reagents.
• Compact & Scalable
  Desktop configuration suitable for mid- to high-throughput research labs.

How It Works (xMAP Technology)

Luminex xMAP technology utilizes color-coded magnetic microspheres, each coated with a capture reagent for a specific target. These beads are mixed with the sample and detection reagents in a liquid phase, allowing rapid binding and reaction kinetics. During acquisition, lasers excite both the bead-specific dye (identification) and the reporter dye (quantification). The system collects data from thousands of microspheres per analyte per sample, ensuring high accuracy and statistical reliability.

Applications

• Multiplex Protein Analysis: Cytokines, chemokines, biomarkers
• Gene Expression Profiling: mRNA quantification in complex samples
• Biomarker Discovery: Disease-related signature analysis
• Drug Screening: Multiplexed assay platforms for pathway analysis
• Immunology Research: Immune profiling and signaling pathway studies
• Clinical Research: Preclinical validation of diagnostic markers (RUO only)

System Contents

• Luminex 200 Instrument
• xMAP Sheath Fluid Delivery System
• X-Y Platform for 96-Well Plates
• Sheath and Waste Bottles
• Sample Probe Needle and Adjustment Kit
• Heater Block (96-well plate compatible)
• Automated Maintenance Plate
• Dell Desktop PC with Windows® 10, Monitor, Keyboard & Mouse
• xPONENT Software (3-seat license + 90-day trial of optional modules: Security, 21 CFR Part 11, Automation, and LIS Integration)
• Documentation

Shipped Separately

• Luminex 200 Calibration Kit
• Luminex 200 Performance Verification Kit

Specifications Table

The Luminex™ 200 Instrument System offers a powerful, flexible solution for laboratories looking to expand their capabilities in multiplexed gene and protein expression analysis. With proven xMAP technology, high throughput, and compatibility with widely used Invitrogen™ assays, it provides reproducible, efficient results for a wide range of biological and translational research applications.