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Applied Biosystems™ PowerTrack™ SYBR Green Master Mix for qPCR
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Specifications:
| Application | qPCR | ||
| Storage Temperature | -20°C ±5°C in the dark | ||
| Product Type | Real Time PCR SYBR Master Mix | Forms | Freeze Dried |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Applied Biosystems™ PowerTrack™ SYBR Green Master Mix is a pre-formulated, optimized 2X master mix designed for highly reproducible real-time PCR (qPCR) using SYBR™ Green detection chemistry. Engineered for maximum specificity and workflow confidence, it incorporates built-in visual dye indicators, antibody-mediated hot-start Taq polymerase, and UNG/dUTP contamination control.
PowerTrack™ SYBR Green Master Mix enables accurate gene expression analysis and microbial detection when used with user-supplied primers and DNA or cDNA templates.
Key Features
- 2X universal SYBR Green qPCR master mix
- Built-in visual blue dye for setup visibility
- Optional yellow sample buffer (color change blue → green upon template addition)
- Antibody-mediated hot-start Taq DNA polymerase
- UNG/dUTP system for carryover contamination prevention
- Compatible with fast and standard cycling modes
- Broad primer Tm compatibility (300–800 nM recommended)
- Tight Ct reproducibility across wide dynamic range
Built-in Visual Indicators
- Blue inert dye enhances visibility in tubes/wells without interfering with fluorescence detection.
- 40X Yellow Sample Buffer (optional) creates a visible color shift from blue to green after sample addition—reducing pipetting errors.
Performance & Specificity
- Hot-start polymerase prevents nonspecific amplification
- High inhibitor tolerance
- Stable assembled reaction for up to 8 hours
- Optimized for challenging qPCR applications
Contamination Control
The incorporation of UNG (Uracil-N-Glycosylase) and dUTP:
- Degrades previously amplified PCR products
- Minimizes false positives
- Enhances reliability in high-throughput PCR laboratories
Instrument Compatibility
Compatible with a broad range of real-time PCR platforms, including:
- Applied Biosystems™ 7500, 7900HT, ViiA™ 7
- QuantStudio™ 3, 5, 6 Flex, 6 Pro, 7 Flex, 7 Pro, 12K Flex
- StepOne™, StepOnePlus™
- Bio-Rad CFX96™, CFX384™, iQ™5
- Roche LightCycler™ 480
- Agilent Mx3005P™
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Chemistry | SYBR Green |
| Concentration | 2X |
| Polymerase | Taq DNA Polymerase (Hot-Start) |
| Passive Reference | ROX (Pre-mixed) |
| Includes | dUTP, UNG |
| Multiplex Capability | Single-plex |
| Reaction Speed | Fast or Standard |
| Sample Type | cDNA or gDNA |
| Shipping | Dry Ice |
| Storage | -20°C (protected from light) |
| Applications | Gene Expression, Microbial Detection |
Available Sizes
| Catalog Number | Quantity |
|---|---|
| A46012 | 1 mL |
| A46109 | 5 mL |
| A46110 | 2 × 5 mL |
| A46111 | 5 × 5 mL |
| A46112 | 10 × 5 mL |
| A46113 | 50 mL |
Applied Biosystems™ PowerTrack™ SYBR Green Master Mix delivers enhanced workflow confidence, contamination control, and high specificity in real-time PCR applications. Its innovative visual dye system simplifies setup while hot-start chemistry and UNG protection ensure reliable amplification.
For laboratories performing gene expression analysis or microbial detection across diverse qPCR platforms, PowerTrack™ SYBR Green Master Mix provides a robust, flexible, and high-performance solution.
- Pack Size: 1 x 1 mL, sufficient for 100 Rxns 1 x 5 mL, sufficient for 500 Rxns 2 x 5 mL, sufficient for 1000 Rxns 5 x 5 mL, sufficient for 2500 Rxns 10 x 5 mL, sufficient for 5000 Rxns 1 x 50 mL, sufficient for 5000 Rxns