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Apollo Scientific Agarose low EEO type D1, Molecular Biology Grade
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Specifications:
| Application | Gel Electrophoresis | ||
| Storage Temperature | Ambient | ||
| Product Type | Molecular Biology Reagent | Forms | Powder |
| Product Brand | Apollo Scientific | ||
| Product Grade | Molecular Biology | ||
Apollo Scientific™ Agarose Low EEO Type D1 is a high-purity molecular biology grade agarose formulated for routine nucleic acid electrophoresis and analytical applications. It offers excellent gel strength, high clarity, and low electroendosmosis (EEO), ensuring optimal separation of DNA and RNA fragments with minimal band distortion.
This agarose is specifically refined for molecular biology applications where DNAse and RNAse contamination must be avoided. It provides reproducible gel matrices suitable for a broad range of electrophoretic analyses, including genomic DNA, PCR products, and plasmid preparations.
Key Features
- Low EEO (0.05–0.13): Minimizes electroendosmotic flow for sharper, more defined bands.
- High Gel Strength: ≥1200 g/cm² at 1% and ≥2400 g/cm² at 1.5%, ensuring easy gel handling and casting.
- Optimal Gelling and Melting Points: Gelling at 36 ± 1.5 °C and melting at 88 ± 1.5 °C for fast solidification and convenient remelting.
- High Clarity (np < 40): Produces transparent gels ideal for imaging and analysis.
- Ultra-Low Impurities: Moisture < 7%, Ash < 0.4%, Sulphate < 0.14%.
- DNase/RNase-free: Certified absence of nucleases ensures reliable molecular biology results.
- Reproducible Performance: Consistent lot-to-lot quality supporting quantitative and qualitative electrophoresis.
- Molecular Biology Grade: Suitable for sensitive downstream applications such as blotting, cloning, and fragment recovery.
Typical Applications
- Routine agarose gel electrophoresis of DNA and RNA
- Analysis of PCR, restriction digest, and plasmid products
- Preparation of gels for Southern and Northern blotting
- DNA purification and fragment recovery
- Molecular cloning workflows
Chemical and Physical Properties
| Property | Specification |
|---|---|
| Gel Strength (1%) | ≥ 1200 g/cm² |
| Gel Strength (1.5%) | ≥ 2400 g/cm² |
| Gelling Temperature | 36 ± 1.5 °C |
| Melting Temperature | 88 ± 1.5 °C |
| Moisture Content | < 7 % |
| Ash Content | < 0.4 % |
| EEO (Electroendosmosis) | 0.05 – 0.13 |
| Sulphate Content | < 0.14 % |
| Clarity (np) | < 40 |
| DNase/RNase Activity | Not detected |
| CAS Number | 9012-36-6 |
| MDL Number | MFCD00081294 |
| EINECS Number | 232-731-8 |
| Storage Temperature | Ambient |
| Grade | Molecular Biology Grade |
Ordering Information
| Catalog Number | Pack Type | Packaging | Pack Size |
|---|---|---|---|
| BIA1177-100G | Bottle | 100 g | Molecular Biology Grade |
| BIA1177-500G | Bottle | 500 g | Molecular Biology Grade |
| BIA1177-1KG | Bottle | 1 kg | Molecular Biology Grade |
Safety Information
- Hazard Classification: Irritant
- Storage Conditions: Store at ambient temperature, tightly sealed in a dry environment.
- Handling: Avoid inhalation or contact with skin and eyes. Use protective gloves and safety goggles during preparation and gel casting.
Apollo Scientific™ Agarose Low EEO Type D1 delivers high mechanical strength, low background interference, and nuclease-free purity, making it an ideal medium for accurate nucleic acid separation and visualization. Its consistent performance, ease of use, and purity standards make it a preferred agarose choice for molecular biology and electrophoresis laboratories.
- Size: 100g 500g 1kg