Apollo Sci. Tetracycline hydrochloride powder, Cell culture tested

Tetracycline hydrochloride is a broad-spectrum, bacteriostatic antibiotic with efficacy against both gram-positive and gram-negative bacteria. It works by inhibiting bacterial protein synthesis, making it highly valuable in research and cell culture applications. The product is cell culture tested, ensuring suitability for laboratory applications that require stringent quality and purity.

Chemical and Physical Properties:

• Chemical Formula: C₂₂H₂₅ClN₂O₈
• Molecular Weight: 480.9 g/mol
• Melting Point: 220–223°C
• MDL Number: MFCD00078142
• CAS Number: 64-75-5

Features:

1. Broad-Spectrum Antibiotic:
   • Effective against a wide range of gram-positive and gram-negative bacteria.
   • Inhibits bacterial growth by binding to the 30S ribosomal subunit, preventing protein synthesis.
2. High Purity:
   • Suitable for use in sensitive biological assays and research.
3. Cell Culture Tested:
   • Ensures that the product is non-toxic to eukaryotic cells when used at recommended concentrations.
   • Ideal for maintaining contamination-free cell cultures.
4. Powder Form:
   • Easy to dissolve and prepare solutions for various applications.
5. Storage:
   • Requires freezer storage (below -20°C) to maintain stability and effectiveness.

Applications:

1. Microbial Research:
   • Study of bacterial protein synthesis inhibition.
   • Analysis of bacterial growth patterns and antibiotic resistance mechanisms.
2. Cell Culture:
   • Prevents bacterial contamination in cell culture experiments.
   • Essential for maintaining aseptic conditions in tissue culture studies.
3. Genetic Engineering:
   • Used as a selection agent in experiments involving tetracycline-resistant bacterial strains.
4. Antibiotic Sensitivity Testing:
   • Determining bacterial susceptibility to tetracycline.
5. Molecular Biology:
   • Supports studies on ribosomal function and translational control.

Usage Guidelines:

1. Preparation:
   • Dissolve the powder in sterile water or buffer to prepare working solutions.
   • Recommended working concentration depends on the application (commonly 10–50 µg/mL for cell culture use).
2. Storage:
   • Store the powder at freezer temperatures (below -20°C) to prevent degradation.
   • Prepared solutions should be stored in aliquots at -20°C to maintain potency.
3. Handling:
   • Handle with care using appropriate personal protective equipment (PPE) due to potential health risks.
   • Avoid prolonged exposure to light, as tetracycline is light-sensitive.

Safety Information:

• Tetracycline can cause photosensitivity and gastrointestinal disturbances.
• Long-term or high-concentration exposure may lead to toxicity or resistance development.
• Proper disposal methods should be followed to prevent environmental contamination.

Apollo Sci. Tetracycline Hydrochloride Powder is a versatile and high-quality bacteriostatic antibiotic designed for rigorous research applications. Its broad-spectrum activity, combined with cell culture testing, makes it a reliable choice for microbiology, cell culture, and molecular biology studies.