Apollo Sci. IPTG (isopropyl-β-D-thiogalactopyranoside) ≥99% dioxane-free

IPTG is a high-purity reagent widely used in molecular biology, specifically for inducing expression in cloning experiments and identifying recombinant colonies. Its dioxane-free formulation ensures compatibility with sensitive applications.

Key Features

1. High Purity:
   • Purity of ≥99%, ensuring reproducibility in molecular biology workflows.
2. Dioxane-Free:
   • Free of dioxane contaminants, making it ideal for critical and sensitive experiments.
3. Colorimetric Detection:
   • Used alongside X-Gal in blue/white screening for visual identification of recombinants in cloning strategies.

Chemical and Physical Properties

Applications

1. Gene Expression:
   • IPTG is used as an inducer for the lac operon in bacterial systems.
   • Induces expression of genes under the control of lac operator/promoter systems.
2. Cloning and Screening:
   • Combined with X-Gal for blue/white screening to differentiate between recombinant (white) and non-recombinant (blue) colonies in bacterial transformation.
3. Protein Production:
   • Induces high levels of recombinant protein expression in systems using IPTG-inducible promoters.
4. Molecular Biology Research:
   • Widely used in experimental setups involving controlled gene expression in bacterial systems.

Advantages

• High Purity: ≥99% assay ensures minimal contaminants for precise molecular biology applications.
• Dioxane-Free: Safe for experiments requiring minimal chemical interference.
• Reliable Induction: Proven effectiveness in systems using the lac operon for controlled gene expression.
• Stable and Easy to Use: Melting point of 105 °C and storage at 2–8 °C ensure stability.

Specifications

Storage and Handling

• Storage Temperature: Store in a refrigerator (2–8 °C) to maintain stability.
• Packaging: Supplied in tightly sealed containers to prevent moisture absorption.
• Shelf Life: Stable under recommended storage conditions.

Safety Information

• Hazard Classification: Irritant; avoid contact with skin and eyes.
• Precautions:
  • Use gloves, goggles, and protective clothing when handling.
  • Handle in a well-ventilated area to avoid inhalation of powder.
• Disposal: Dispose of in accordance with local chemical waste regulations.

Apollo Scientific IPTG (Isopropyl-β-D-thiogalactopyranoside) is a high-purity, dioxane-free reagent tailored for gene expression and blue/white screening in molecular biology research. Its reliability and compatibility with sensitive experiments make it a critical tool for bacterial transformation and protein production workflows.